DCBLD1基因对胃癌细胞增殖、迁移和自噬的影响及分子机制研究
收稿日期: 2025-10-09
修回日期: 2026-01-02
录用日期: 2026-02-06
网络出版日期: 2026-04-21
基金资助
中央引导科技发展资金项目（ZYYD2024JD05）；新疆维吾尔自治区研究生创新项目（XJ2025G181）
Study on the Effects of DCBLD1 on Proliferation， Migration， and Autophagy in Gastric Cancer Cells and Its Molecular Mechanisms
Received date: 2025-10-09
Revised date: 2026-01-02
Accepted date: 2026-02-06
Online published: 2026-04-21
目的：探讨DCBLD1基因对胃癌细胞增殖、迁移及自噬的影响及分子机制。方法：通过生物信息学分析DCBLD1基因在泛癌及胃癌中信使核糖核酸（mRNA）的表达情况以及对胃癌诊断的预测价值；采用蛋白印迹法检测胃癌与正常胃黏膜细胞中DCBLD1蛋白表达水平。构建DCBLD1敲低质粒，转染AGS、NUGC3细胞后，采用蛋白质印迹法检测其转染效果以及对LC3、P62表达的影响。通过CCK8、Transwell实验以及克隆形成实验检测细胞增殖及迁移能力。结果：生物信息学分析结果提示，DCBLD1在多种肿瘤中高表达，在27对胃癌配对样本分析结果显示，DCBLD1在胃癌组织中的表达量高于正常组织中的表达量。DCBLD1对胃癌有较高的诊断价值。Kaplan Meier Plotter数据库生存分析结果显示，DCBLD1高表达的胃癌患者预后明显差于低表达患者。Western Blot实验结果显示，与正常胃黏膜细胞GES-1相比，胃癌细胞株AGS、NUGC3中DCBLD1蛋白表达水平明显升高。Western Blot实验检测敲低结果提示，敲低DCBLD1的表达后LC3表达升高、p62表达下降。CCK8实验结果显示，敲低DCBLD1的表达能够减弱胃癌细胞的增殖能力。Transwell实验结果显示，敲低DCBLD1的表达能够减弱胃癌细胞的迁移能力。克隆形成实验结果显示，敲低DCBLD1的表达能够减弱胃癌细胞集落形成能力，而敲低DCBLD1后用自噬抑制剂3-MA可以部分逆转胃癌细胞的增殖作用。结论：DCBLD1在胃癌细胞中呈现高表达，并且能促进胃癌细胞增殖、迁移能力，可能与DCBLD1抑制细胞自噬过程有关。
来比江·吾斯曼
,
张文斌
.
DCBLD1基因对胃癌细胞增殖、迁移和自噬的影响及分子机制研究
Objective： To investigate the effects of DCBLD1 on the proliferation， migration， and autophagy of gastric cancer cells and explore its underlying molecular mechanisms.Methods： Bioinformatics analysis was performed to examine the mRNA expression levels of DCBLD1 in pan-cancer and gastric cancer， as well as its predictive value for gastric cancer diagnosis. Western blotting was used to detect DCBLD1 protein expression in gastric cancer cells and normal gastric mucosal cells. DCBLD1 knockdown plasmids were constructed and transfected into AGS and NUGC3 cells， followed by Western blotting to assess transfection efficiency and its effects on LC3 and P62 expression. Cell proliferation and migration abilities were evaluated using CCK-8， Transwell， and colony formation assays.Results： Bioinformatics analysis revealed that DCBLD1 is highly expressed in multiple cancers. Analysis of 27 paired gastric cancer samples showed that DCBLD1 expression was significantly higher in gastric cancer tissues than in normal tissues. DCBLD1 demonstrated high diagnostic value for gastric cancer. Survival analysis in the Kaplan-Meier Plotter database indicated that gastric cancer patients with high DCBLD1 expression had significantly worse prognosis than those with low expression. Western blot results showed that DCBLD1 protein levels were markedly elevated in gastric cancer cell lines （AGS and NUGC3） compared to the normal gastric mucosal cell line GES-1. Knockdown of DCBLD1 increased LC3 expression and decreased P62 expression. CCK-8 assays demonstrated that DCBLD1 knockdown inhibited gastric cancer cell proliferation. Transwell assays revealed that DCBLD1 silencing impaired gastric cancer cell migration. Colony formation assays indicated that DCBLD1 knockdown reduced the clonogenic ability of gastric cancer cells， and 3-MA partially reversed the proliferative effects induced by DCBLD1 downregulation.Conclusion： DCBLD1 is highly expressed in gastric cancer cells and promotes their proliferation and migration. This effect may be associated with DCBLD1-mediated inhibition of autophagy.
Key words： Stomach cancer； ; Autophagy； ; DCBLD1 gene； ; Cell proliferation； ; Cell migration
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