CHINESE JOURNAL OF MEDICINAL GUIDE >

2025 , Vol. 27 >Issue 5: 497 - 506

DOI: https://doi.org/magtech.2025.02.14-00001

Comparison of Time-Concentration Characteristics of Active Ingredients from Yixinyin in Plasma and Heart Samples of Normal and Atrial Fibrillation Rats by LC-MS

  • Juan LI ,
  • Feng 无 ZHANG
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  • 1.Anhui University of Chinese Medicine，Anhui Hefei 230012， China；
    2.Department of Pharmacya， Department of Rehabilitation Medicineb， Changzheng Hospital，
    Naval Medical University， Shanghai 200003， China；
    3.Department of Pharmacy， Zhabei Center Hospital， Jing'an District， Shanghai 200070， China；
    4 Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China

Received date: 2025-02-14

  Revised date: 2025-04-28

  Accepted date: 2025-06-09

  Online published: 2025-07-23

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Abstract

Objective： To investigate the time-concentration characteristics of the plasma and cardiac migratory components of Yixinyin in normal and atrial fibrillation rats based on liquid chromatography-mass spectrometry technology.Methods： Rats were fed with an alcohol-liquid diet for 30 consecutive days， and left atrial dilation was confirmed by echocardiography. Subsequently， atrial fibrillation （AF） model was induced by continuous 7-day tail vein injection of acetylcholine-calcium chloride. Rats were randomly divided into four groups： normal group， model group， normal+ Yixinyin group， and model+Yixinyin group. Yixinyin was administered at a dose of 31 g·kg-1·d-1， while non-treated two groups received an equivalent volume of saline. After 14 consecutive days of intragastric administration， whole blood samples were collected at multiple time points， and cardiac tissues were harvested at the endpoint. Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was employed to detect the migrating components in the tested samples.Results： Compared with the normal group， the rate of atrial fibrillation induction， susceptibility to atrial fibrillation， and duration of atrial fibrillation were significantly higher in the model group （P < 0.05）， suggesting successful modeling. In vitro， 111 chemical components were identified in Yixinyin， and 27 and 8 chemical components were detected in plasma and heart of normal and atrial fibrillation rats， respectively. The time-concentration profile results indicated that 4-hydroxycinnamic acid，vanillin，salicylic acid B， ononin， daidzein， calycosin， isoliquiritigenin， myristicin， senkyunolide A， and glycyrrhetinic acid were present at higher levels in the plasma of AF rats. Additionally， eight components， including liquiritin， calycosin 7-glucoside， and gingerol， were also detected in heart tissue species.Conclusion： The targeted cardiac components， namely liquiritin， calycosin-7-glucoside， and gingerol， may serve as the key pharmacologically active constituents of Yixinyin in the treatment of AF.


Key words： Yixinyin； ; Atrial fibrillation； ; UHPLC-Q-TOF/MS； ; Liquiritin； ; Calycosin 7-glucoside； ; Gingerol

Cite this article

Juan LI , Feng 无 ZHANG .

Comparison of Time-Concentration Characteristics of Active Ingredients from Yixinyin in Plasma and Heart Samples of Normal and Atrial Fibrillation Rats by LC-MS

[J]. CHINESE JOURNAL OF MEDICINAL GUIDE, 2025 , 27(5) : 497 -506 . DOI: magtech.2025.02.14-00001

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