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Correlation Analysis between the Distribution of Uterine Microbiota and the Outcome of Frozen-Thawed Embryo Transfer in Infertile Patients
Received date: 2025-04-30
Revised date: 2025-07-23
Accepted date: 2025-11-15
Online published: 2025-12-24
Objective： To explore the correlation between the distribution of uterine microbiota and the outcome of frozen-thawed embryo transfer in infertile patients.Methods： 60 infertile patients who underwent frozen-thawed embryo transfer treatment in our hospital from January to December 2024 were selected， including 32 cases with clinical pregnancy （pregnanty group） and 28 cases without clinical pregnancy （non pregnanty group）. The differences in clinical general information and uterine microbiota between two groups of patients were analyzed.Results： There was no statistically significant difference in clinical general data such as age， body mass index， and infertility type between the pregnant group and the non pregnant group （P>0.05）. Microbial analysis showed that there were 780 operational taxonomic units （OTUs） unique to the pregnant group， 1355 OTUs unique to the non pregnant group， and 987 OTUs shared by the two groups. There was no statistically significant difference in Shannon index， Simpson index， Sobs index， Ace index， and Chao index of uterine microbiota between the pregnant group and the non pregnant group （P>0.05）. There was no statistically significant difference in microbial community structure （P>0.05）. In terms of bacterial abundance， the pregnant group had a microbiota of lactobacillus 3.30% （2.84， 4.55）， achromobacter 6.70% （4.48， 8.20）， and comamonas 0.22% （0.18， 0.40） in the uterine cavity， respectively， which were higher than those in the non pregnant group （P<0.05）. However， the abundance of aquatic bacteria and psychrophilic bacteria was 0.87% （0.32， 1.22） and 0.05% （0， 0.12）， respectively， which were lower than those in the non pregnant group （P<0.05）.Conclusion： The composition and structure of the uterine microbiota in patients with different outcomes of frozen-thawed embryo transfer are basically similar， but there are differences in the abundance of bacterial genera. The impact of uterine microbiota on pregnancy outcomes may be limited.
Key words： Uterine microbiota； ; Infertility； ; Frozen thawed embryo transfer； ; Pregnancy outcome
ZHOU Wenjing, ZHOU Qi, Chen Qian, CHEN Miaomiao, LIU Qiao, ZHANG Shigui
.
Correlation Analysis between the Distribution of
Uterine Microbiota and the Outcome of Frozen-Thawed Embryo Transfer
in Infertile Patients
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