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Effects of Qiqi Yishen Capsules on Podocyte Pyroptosis in Diabetes Nephropathy Rats Based on the NLRP3/GSDMD Pathway
Received date: 2024-12-05
Revised date: 2025-10-28
Accepted date: 2025-12-24
Online published: 2026-01-26
Objective： To investigate the effect of Qiqi Yishen capsules on inhibiting podocyte pyroptosis through NLRP3/ GSDMD pathway and delaying the progression of diabetic nephropathy.Methods： By establishing diabetes nephropathy （DN） rat model， 48 SD rats were randomly divided into five groups with 12 rats in each group， which were the blank group， the model group， benazepril positive control group， Qiqi Yishen capsule treatment group. Compare and analyze the levels of 24-hour urinary protein， blood urine nitrogen （BUN）， blood creatinine （SCr）， triglyceride （TG） ，total cholesterol （TC） of the rats and renal pathological morphology was observed. IL-1β and IL-18 were detected by ELISA.The expression of GSDMD protein in rat kidney was detected by immunohistochemistry. The expressions of NLRP3， Caspase-1，and Nephrin in renal tissues were detected by Western blot.Results： Compared with the blank group， the levels of urinary protein， blood lipid （TG， TC）、 BUN、 SCr increased in the model group. Meanwhile， there was capillary loop hypertrophy of glomerular tissue in renal tissue. Mesangial matrix increased significantly， and basement membrane thickened. The levels of IL-1β and IL-18 ， and the expressions of GSDMD， NLRP3 and Caspase-1 increased and the expression of podocyte marker Nephrin decreasesd （P<0.01）. Compared with the model group， urine protein， blood lipid， BUN， SCr decreased in the treatment group. At the same time， mesangial matrix decreased. The levels of IL-1β and IL-18 decreased. The expressions of GSDMD， NLRP3 and Caspase-1 decreased， and the level of Nephrin protein recovered in the treatment groups.Conclusion： Qiqi yishen capsules may inhibit podocyte pyroptosis， reduce proteinuria and delay the progression of kidney disease by regulating NLRP3/GSDMD pathway.
Key words： Diabetes nephropathy； ; Pyroptosis； ; NLRP3； ; Qiqi yishen capsules； ; Podocyte
LIU Xinghua, WANG Shirong, ZHANG Jinzhen, GUO Erhua, ZHAO Yang
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Effects of Qiqi Yishen Capsules on Podocyte
Pyroptosis in Diabetes Nephropathy Rats Based on the NLRP3/GSDMD Pathway
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