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Simultaneous Determination of Four Chemical Components in Zephyranthes Candida Flowers by HPLC-QAMS
Southwest Medical University， Sichuan Luzhou 646699， China；
2.Public Center of Experimental Technology，Science and Technology Department， Southwest Medical University， Sichuan Luzhou 646699， China
Received date: 2024-11-14
Revised date: 2025-10-07
Accepted date: 2025-12-24
Online published: 2026-01-26
Objective： To establish a method for simultaneously determining the contents of four chemical components （rutin， isoquercitrin， nicotiflorin and astragalin） in Zephyranthes candida flowers by high performance liquid chromatography combined with the quantitative analysis of multi-components by single marker（HPLC-QAMS）.Methods： The HPLC-QAMS method was adopted. The chromatographic column was Lubex Kromasil C18 （250 mm × 4.6 mm， 5 µm）. The mobile phase was a gradient elution of acetonitrile-0.1% phosphoric acid aqueous solution. The detection wavelength was 350 nm. The flow rate was 1.0 mL·min-1. The column temperature was 30 ℃. The injection volume was 10 µL. Using rutin as the internal reference substance， the relative correction factors of isoquercitrin， nicotiflorin and astragalin relative to rutin were calculated by using the slope correction， multi-point correction and single-point correction methods respectively. The chromatographic peaks were located by the relative retention time. The difference between the results of the QAMS and the external standard method was investigated by the relative error （RE）， and the accuracy and feasibility of the quantitative analysis of multi-components by single marker were evaluated.Results： The linear ranges were 0.279-5.580 µg for rutin， 0.013-0.260 µg for isoquercitrin， 0.208-4.160 µg for nicotiflorin， and 0.019-0.372 µg for astragalin（r ≥ 0.999 9）. The RSDs of the precision， stability and repeatability tests were all less than 2%（n = 6）. The range of the average sample addition recoveries was 97.12% - 98.47% （RSD < 2%， n = 9）. There was no significant difference between the results of the QAMS method using slope correction and multi-point correction and those of the external standard method， and the results of the QAMS method using single-point correction were completely consistent with those of the external standard method.Conclusion： The established content determination method is simple， rapid， accurate and reliable， and can be used to simultaneously determine the contents of the 4 active components in Zephyranthes candida flowers.
YUAN Houlan, YANG Shiyan, HE Bing
.
Simultaneous Determination of Four Chemical
Components in Zephyranthes Candida Flowers
by HPLC-QAMS
［1］ 詹冠群.葱莲，韭莲和长叶野桐的化学成分及生物活性研究［D］.武汉：华中科技大学，2017.
［2］ 南京中医药大学.中药大辞典（上册）（第二版）［M］．上海：上海科学技术出版社，2014：1391．
［3］ 国家中医药管理局《中华本草》编委会.中华本草（第22卷）［M］．上海：上海科学技术出版社，1999：214．
［4］ Xie W， Sun M， Fan J， et al. Transcriptomics and network pharmacology analysis reveal key genes in alkaloid biosynthesis in Zephyranthes candida and therapeutic targets for LIHC［J］.Planta， 2025，262（2）：45.
［5］ Zhan G， Gao B， Zhou J， et al. Structurally diverse alkaloids with nine frameworks from Zephyranthes candida and their acetylcholinesterase inhibitory and anti-inflammatory activities［J］.Phytochemistry， 2023，207：113564.
［6］ Zhan G， Liu J， Zhou J， et al. Amaryllidaceae alkaloids with new framework types from Zephyranthes candida as potent acetylcholinesterase inhibitors［J］. Eur J Med Chem， 2017，127：771-780.
［7］ Zhan G， Qu X， Liu J， et al. Zephycandidine A， the first naturally occurring imidazo［1，2-f］phenanthridine alkaloid from Zephyranthes candida， exhibits significant anti-tumor and anti-acetylcholinesterase activities［J］.Sci Rep， 2016，6：33990.
［8］ Zhan G， Zhou J， Liu R， et al. Galanthamine， plicamine， and secoplicamine alkaloids from Zephyranthes candida and their anti-acetylcholinesterase and anti-inflammatory activities［J］.J Nat Prod， 2016，79（4）：760-766.
［9］ Wei G， Xu Y， Xu MM， et al. Identification of volatile compounds and terpene synthase （TPS） genes reveals ZcTPS02 involved in β-ocimene biosynthesis in Zephyranthes candida［J］.Genes（Basel）， 2024，15（2）：185.
［10］ Chen X， Bi S. Identification of volatile components of flowers of Zephyranthes candida （Lindl.） Herb［J］.Bangl J Bot， 2022，51（1）：175-178.
［11］ 杨诗宇，宋基正，杨山景，等.芦丁药理作用与新剂型的研究进展［J］.中国现代应用药学，2022，39（10）：1360-1370.
［12］ 尚思琦，岳彩芳，陈誉敏.芦丁抗菌作用的研究进展［J］.广东化工，2023，50（24）：70-72.
［13］ 毛雅君，冯亚莉，王梦娇，等.芦丁衍生物的研究进展［J］.中国中药杂志，2021，46（18）：4654-4665.
［14］ 陈燕，詹羽姣，李晨阳，等.异槲皮苷的生物活性研究进展［J］.中国现代中药，2018，20（12）：1579-1582.
［15］ Song B， Niu W， Sun H， et al. Isoquercitrin： from natural source to clinical candidate-synthesis， pharmacology， and metabolic safety［J］.Fitoterapia， 2025，185：106766.
［16］ Patel DK. Medicinal Importance， pharmacological activities and analytical aspects of a flavonoid glycoside 'nicotiflorin' in the medicine［J］.Drug Metab Bioanal Lett， 2022，15（1）：2-11.
［17］ Ruan J， Shi Z， Cao X， et al. Research progress on anti-inflammatory effects and related mechanisms of astragalin［J］.Int J Mol Sci， 2024，25（8）：4476.
［18］ 陈续东，白俊丞，张彬.紫云英苷抗肿瘤作用机制及研究进展［J］.现代医学与健康研究电子杂志，2024，8（20）：130-133.
［19］ 何兵，梁思成，田吉，等.不同校正和定位方法在常春藤一测多评中的应用［J］.药物分析杂志，2023，43（7）：1135-1147.
［20］ 何兵，田吉，杨世艳.一种新的校正方式单点校正在桑叶一测多评中的应用［J］.药物分析杂志，2021，41（7）：1133-1147.
［21］ 梁欣雨，周晓钢，何兵，等.一测多评法同时测定小儿金翘颗粒中7种成分的含量［J］.中国药房，2020，31（10）：1179-1184.
［22］ 白娟，朱倩云，白华，等.高效液相色谱法同时测定桑叶中绿原酸及4种黄酮类成分的含量［J］.中药新药与临床药理，2020，31（4）：469-472.
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