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Impacts of Total Glucosides of Paeonia on the Proliferation， Migration and Oxaliplatin Resistance of Colorectal Cancer Cells by Regulating FAK/MEK/ERK Signaling Pathway
Received date: 2025-05-22
Revised date: 2025-08-04
Accepted date: 2026-02-06
Online published: 2026-02-11
Objective： To explore the impacts of total glucosides of paeonia on the proliferation， migration， and resistance to oxaliplatin （L-OPP） of colorectal cancer cells by regulating focal adhesion kinase （FAK）/mitogen activated protein kinase kinase （MEK）/extracellular regulated protein kinase （ERK） signaling pathway.Methods： Human colorectal cancer HCT116 cells were cultured in vitro and assigned into the control group， low， medium， and high-dose total glucosides of paeonia groups （25， 50， 100 μg·mL-1 total glucosides of paeonia）， and high-dose total glucosides of paeonia+zinc40099027 （Zn27） group （100 μg·mL-1 total glucosides of paeonia+2 nmol·L-1 FAK activator Zn27）. MTT assay was performed to measure cell proliferation. Flow cytometry was used to measure cell apoptosis. Scratch experiment was used to measure cell migration. Transwell method was performed to detect cell invasion. Western blotting （Western blot） was performed to detect the proteins related to the FAK/MEK/ERK signaling pathway. The human colorectal cancer drug-resistant cell line HCT116/Oxaliplatin （L-OPH） was constructed and divided into L-OPH-control group， L-OPH-low， medium， and high dose total glucosides of paeonia groups， and L-OPH-high dose total glucosides of paeonia+Zn27 group. The MTT assay was used to detect drug resistance in HCT116/L-OPH cells.Results： Compared with the control group， the low-dose total glucosides of paeonia group， medium-dose total glucosides of paeonia group， and high-dose total glucosides of paeonia group showed decreased in cell survival rate， scratch healing rate， number of invasive cells， p-FAK/FAK， p-MEK1/2/MEK1/2， p-ERK1/2/ERK1/2 proteins， and increased cell apoptosis rate （P<0.05）. Compared with the high-dose total glucosides of paeonia group， the high-dose total glucosides of paeonia+Zn27 group showed increased cell survival rate， scratch healing rate， number of invasive cells， p-FAK/FAK， p-MEK1/2/MEK1/2， and p-ERK1/2/ERK1/2 proteins， and decreased cell apoptosis rate （P<0.05）. The L-OPH-low， medium， and high-dose total glucosides of paeonia groups showed decreased cell survival rate than the L-OPH-control group （P<0.05）. The L-OPH-high-dose total glucosides of paeonia+Zn27 group had higher cell survival rate than the L-OPH-high-dose total glucosides of paeonia group （P<0.05）.Conclusion： total glucosides of paeonia may inhibit the proliferation， migration， and L-OPH resistance of colorectal cancer cells by suppressing FAK/MEK/ERK signaling pathway.
TU Jiajia, WANG Liyun, SUN Bo, WANG Fanrong
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Impacts of Total Glucosides of Paeonia on the
Proliferation， Migration and Oxaliplatin Resistance of
Colorectal Cancer Cells by Regulating FAK/MEK/ERK Signaling Pathway
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