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Study on the Effects of DCBLD1 on Proliferation， Migration， and Autophagy in Gastric Cancer Cells and Its Molecular Mechanisms
Received date: 2025-10-09
Revised date: 2026-01-02
Accepted date: 2026-02-06
Online published: 2026-04-21
Objective： To investigate the effects of DCBLD1 on the proliferation， migration， and autophagy of gastric cancer cells and explore its underlying molecular mechanisms.Methods： Bioinformatics analysis was performed to examine the mRNA expression levels of DCBLD1 in pan-cancer and gastric cancer， as well as its predictive value for gastric cancer diagnosis. Western blotting was used to detect DCBLD1 protein expression in gastric cancer cells and normal gastric mucosal cells. DCBLD1 knockdown plasmids were constructed and transfected into AGS and NUGC3 cells， followed by Western blotting to assess transfection efficiency and its effects on LC3 and P62 expression. Cell proliferation and migration abilities were evaluated using CCK-8， Transwell， and colony formation assays.Results： Bioinformatics analysis revealed that DCBLD1 is highly expressed in multiple cancers. Analysis of 27 paired gastric cancer samples showed that DCBLD1 expression was significantly higher in gastric cancer tissues than in normal tissues. DCBLD1 demonstrated high diagnostic value for gastric cancer. Survival analysis in the Kaplan-Meier Plotter database indicated that gastric cancer patients with high DCBLD1 expression had significantly worse prognosis than those with low expression. Western blot results showed that DCBLD1 protein levels were markedly elevated in gastric cancer cell lines （AGS and NUGC3） compared to the normal gastric mucosal cell line GES-1. Knockdown of DCBLD1 increased LC3 expression and decreased P62 expression. CCK-8 assays demonstrated that DCBLD1 knockdown inhibited gastric cancer cell proliferation. Transwell assays revealed that DCBLD1 silencing impaired gastric cancer cell migration. Colony formation assays indicated that DCBLD1 knockdown reduced the clonogenic ability of gastric cancer cells， and 3-MA partially reversed the proliferative effects induced by DCBLD1 downregulation.Conclusion： DCBLD1 is highly expressed in gastric cancer cells and promotes their proliferation and migration. This effect may be associated with DCBLD1-mediated inhibition of autophagy.
Key words： Stomach cancer； ; Autophagy； ; DCBLD1 gene； ; Cell proliferation； ; Cell migration
LAI Bijiang•Wusiman , SONG Dingding, ZHANG Wenbin
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Study on the Effects of DCBLD1 on Proliferation， Migration， and Autophagy in
Gastric Cancer Cells and Its Molecular Mechanisms
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