酶联免疫斑点法和ELISA法诊断结核病临床价值的探讨

酶联免疫斑点法和ELISA法诊断结核病临床价值的探讨

石海萍,周晓芹

陕西省结核病防治院内七科；2陕西省旬邑县医院感染科

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-07-25

Clinical Value of ELISPOT and ELISA Method in Diagnosis of Tuberculosis

Shi Hai-ping,Zhou Xiao-qin

Department of Internal Seven, Shanxi Tuberculosis Prevention and Control Hospital; 2Department of Infectious Disease, Xunyi County Hospital of Shanxi Province

Received:1900-01-01 Revised:1900-01-01 Online:2016-07-25

摘要/Abstract

摘要： 【摘要】目的：探讨酶联免疫斑点法(ELISPOT)和ELISA法诊断结核病的临床价值。方法：86例疑似结核病患者，同时行ELISPOT和ELISA法的检验，对结果做配对卡方检验及Kappa一致性检验，比较两种方法的检验的结果，探讨临床诊断应用的价值。结果：配对卡方检验，P值为0.774，＞0.05，不拒绝H0，二者无差异。Kappa一致性检验，Kappa值为0.579，0.4＜Kappa值≤0.75表示一致性中等。结论：两种方法检验结果差异无显着性，具有一致性，结合实验操作性，方便性，ELISA法更适合应用于临床。

Abstract: 【ABSTRACT】Objective: To investigate the clinical value of enzyme linked immunosorbent assay (ELISPOT) and ELISA method in the diagnosis of tuberculosis. Methods: 86 cases of suspected tuberculosis patients. At the same time, the ELISPOT and ELISA test, the paired chi square test and kappa consistency test, to compare the two methods of test results of the value of clinical application. Results:Paired chi square test, P value was 0.774,＞0.05, does not reject H0, no difference between the two.Kappa consistency test, Kappa = 0.579, Kappa＞0.4 or lower than 0.75 medium consistency.Conclusion:There were no significant differences between the two methods. The results showed that the ELISA method was more suitable for clinical application.

石海萍,周晓芹. 酶联免疫斑点法和ELISA法诊断结核病临床价值的探讨[J]. .

Shi Hai-ping,Zhou Xiao-qin. Clinical Value of ELISPOT and ELISA Method in Diagnosis of Tuberculosis[J]. .

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