Abstract: Objective： To provide scientific evidence for the establishment of AIDS prevention and control through analyzing the situation and influencing factors of HIV / AIDS counseling and testing in medical institutions in Beijing. Methods：According to HIV testing and counseling quarterly tables in 2016，We summarized and analyzed the datas of AIDS counseling and testing work. At the same time， We analyzed the influencing factors by questionnaire in PITC work. Results：4 052 235 HIV antibody were detected by 151 medical institutions.2 942 positive HIV-infected were found and positive rate was 0.73‰. The detection of HIV in medical institutions of higher proportion of the department were venereal outpatient department and inpatient department and andrology， respectively the detecting rate was 71.17%， 50.06% and 28.23%.The higher positive rate of HIV detection were STD clinic and department of infection， respectively the positive rate were 9.75‰ and 6.52‰. The main influencing factors of PITC in medical institutions were that cases unable to cooperate， high charge and lack of publicity，Respectively accounting for 84.77%， 40.40% and 38.41%. Conclusion：By further publicity， so that people understood the importance of AIDS testing. AIDS training should be strengthened for medical staff in key departments to promote PITC work.