Abstract: Objective： To investigate the method of intracerebroventricular administration of conscious rats without anesthesia. Methods：15 femaleSD rats were selected.The tube was placed in the lateral ventricles by the self-made ventricular catheterization system. After the catheterization, the rats recovered freely for 1 week, and the rats were tamed for 5 to 10 minutes every day. From 8 to 10 days after operation,10 μL physiological saline was injected into the lateral ventricle every day without anesthesia. On the 11th day rats were anesthetized after 10 μL intracerebroventricular injection of methylene blue, open all chest and the rats were sacrificed by formaldehydeperfusion though the ascending aorta cannulation. After that the brain tissues were took out. Results：All inner wall of ventricle were stained after 2% methylene blue was poured in. There was no obvious bleeding or inflammation after hematoxylin and eosin staining (HE staining) in the brain, There was no cerebrospinal fluid in stainless steel conduit placed in the lateral ventricle catheter of 2 rats before insertion.Conclusion：The method of intracerebroventricular administration of rats without anesthesia is simple and feasible, and the rate of success is high.