紫外分光光度法测定灵芝孢子油中总三萜含量

紫外分光光度法测定灵芝孢子油中总三萜含量

宋福江,王玉乐,闫寒,范斌,孙健

中国中医科学院医学实验中心,中科乐仁北京科技发展有限公司,中国中医科学院医学实验中心,中国中医科学院医学实验中心,中国中医科学院医学实验中心

收稿日期:2017-09-10 修回日期:2017-09-12 出版日期:2017-09-25
基金资助:
国家自然科学基金资助项目（项目编号：81102672；项目名称：基于耐药转运蛋白的前列腺屏障相关引使药配伍机制研究）；中央级公益性科研院所基本科研业务费专项资金(项目编号：ZZ2016013，ZZ2015016；项目名称：中药复方抗菌作用的网络药理学研究，实验室分析检测平台能力与认证体系的示范性研究)；中央本级重大增减支项目(项目编号：2060302；项目名称：基于超高效液相-飞行时间质谱技术的中药代谢组学研究)

Determination of Total Triterpenoids in Ganoderma Lucidum Spores Oil by Ultraviolet Spectrophotometry

SONG Fu-jiang,WANG Yu-le,YAN Han,FAN Bin and SUN Jian

Experimental Research Center,China Academy of Chinese Medical Sciences,State Key Laboratory Breeding Base of Dao-di Herbs,Zhongke Leren Science#$NBSTechnology Development CO,LTD,Experimental Research Center,China Academy of Chinese Medical Sciences,State Key Laboratory Breeding Base of Dao-di Herbs,Experimental Research Center,China Academy of Chinese Medical Sciences,State Key Laboratory Breeding Base of Dao-di Herbs,Experimental Research Center,China Academy of Chinese Medical Sciences,State Key Laboratory Breeding Base of Dao-di Herbs

Received:2017-09-10 Revised:2017-09-12 Online:2017-09-25

摘要/Abstract

摘要： 目的：建立紫外分光光度法测定灵芝孢子油中总三萜含量。方法：以齐墩果酸为对照品，与5%香草醛-冰乙酸反应，在高氯酸环境下显色，在545 nm处测定样品吸光度。结果：齐墩果酸在0.02~0.12 mg范围内线性关系良好，Y=11.033X-0.0441，r=0.9998。重复性实验，RSD为2.84%（n=6）。平均回收率78.43%，RSD为3.5%（n=6）。结论：经过线性、重复性、精密度、回收率实验，确认本方法适用于灵芝孢子油总三萜的含量测定。

Abstract: Objective：To establish a method for determining the content of total triterpenoids in the ganoderma lucidum spores oil by ultraviolet spectrophotometry. Methods：Ultraviolet spectrophotometry determination was performed on oleanolic acid (as a control) and with 5% vanillin-glacial acetic acid solution and perchloricacid ascolor-developing agent.The absorbance of sample was determined at 545nm. Results：There was a good linear relationship between the concentration and absorbance of oleanolic acid within the range of 0.02~0.12 mg(A=11.033c-0.0441，r=0.9998).The repeat test，RSD=2.84%(n=6)，the average recovery rate was 78.43%，RSD=3.5%(n=6).Conclusion：After the linearity， the repeatability， the precision， the recovery of the test，the method can be used for measuring the content of total triterpenoid in the ganoderma lucidum spores oil.

宋福江,王玉乐,闫寒,范斌,孙健. 紫外分光光度法测定灵芝孢子油中总三萜含量[J]. .

SONG Fu-jiang,WANG Yu-le,YAN Han,FAN Bin and SUN Jian. Determination of Total Triterpenoids in Ganoderma Lucidum Spores Oil by Ultraviolet Spectrophotometry[J]. .

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