Abstract: Objective：To evaluate and analyze quality of the clinical practice guidelines of stroke， in order to give reference for clinical practice. Methods：We systematically searched the five general databases， including PubMed， EMBASE， Chinese Biomedical Literature Database (CBM)， Wanfang， and China National Knowledge Infrastructure (CNKI)， and four guidelines databases， namely， Guidelines International Network（GIN）， Scottish Intercollegiate Guidelines Network（SIGN）， National Guideline Clearinghouse（NGC）and National Institute for Health and Clinical Excellence（NICE）. Additionally， we also searched the website of Yimaitong.Two independent reviewers screened and extracted data. When meeting the disagreement， we solved it by discussion or consultation with a third reviewer.Two reviewers evaluated quality of the included guidelines by AGREE Ⅱ instrument.The consistency was assessed by the ICC value. Results：A total of 11 086 records were retrieved from the databases.Finally， 22 guidelines were included after two phases of screening.These guidelines were published from 2008 to 2016， and mainly from seven countries， particularly China and UK (six guidelines， 27.3%). Six guidelines (27.3%) were from the guidelines databases. Of these， five guidelines were from NICE.The consistency between two assessors was high， and ICC value was 0.913 [95%CI(0.862， 0.933)， P=0.009]. The scores of six domains were scope and purpose（45.3%， SD=18.6%）， stakeholder and involvement（20.5%， SD=14.8%）， rigor of development（30.8%， SD=17.5%）， clarity of presentation（62.6%， SD=18.7%）， applicability（14.6%， SD=9.0%）， editorial independence（31.3%， SD=21.4%）， respectively.The sub-group analyses showed that there was little influence on quality of the included guidelines in publication year， publication type， and guidelines type， while the reporting of funding and update， the search of evidence were the risk factors which apparently influenced the scores of the domains of “clarity of presentation” and“editorial independence”.Meanwhile， Chinese stroke guidelines scored lower than the guidelines from other countries in the domains of “stakeholder and involvement”and “editorial independence”. Conclusion：The majority of stroke guidelines are from developed countries in European and American.By comparing with the average scores of the guidelines worldwide， the scores of stroke guidelines are similar in the domains of “clarity of presentation”and “editorial independence”， but apparently lower in the remaining domains.