Abstract: Objective： To investigate the distribution and drug resistance of pathogens causing bloodstream infections in hospitalized patients，and provide evidence for rational clinical use of antibiotics and control of healthcare-associated bloodstream infection. Methods：From June 2012 to June 2018，the pathogenic bacteria isolated from blood culture and antimicrobial susceptibility testing results in this hospital were analyzed retrospectively. Results： A total of 494 strains of pathogenic bacteria were isolated from 7 576 blood culture specimens，the positive rate was 6.52%. The top five pathogens were Klebsiella pneumoniae （n=88，17.8%），Escherichia coli（n=84，17.0%），Staphylococcus aureus（n=61，12.3%）， Staphylococcus epidermidis（n=59，11.9%）and Enterococcus faecalis（n=23，4.7%），including 276 gram negative bacilli（55.9%），190 gram positive cocci（38.5%）， and 28 fungus（5.7%）．The resistant rates of enterobacteriaceae bacteria to meropenem，imipenem，piperacillin/tazobactam and cefopemzone/sulbactam were relatively low（1.2%-17.0%）. The detection rate of methicillin-resistant S. aureus（MRSA） and methicillin-resistant S. epidermidis（MRSE） were 57.4% and 71.2% respectively. There were no strains of main gram-positive bacteria that were found to be resistant to macrodantin、 linezolid and vancomycin．The most common fungus in blood culture were Candida albicans（2.6%）and Candida glabrata（1.8%）．Conclusion： Enterobacteriaceae and staphylococcus spp. are the main pathogens causing bloodstream infection in this hospital in recent six years. Early blood culture and surveillance on drug-resistant pathogenic isolates can provide reliable evidence for clinical diagnosis and treatment of bloodstream infection.