Abstract: Objective：To understand the main types and prevalence of carbapenemases in Klebsiella pneumoniae（CR-KP） resistant to carbapenems. Methods：A total of 34 CR-KP strains were collected from our hospital from January 2016 to December 2017， which were insensitive to the results of drug susceptibility test of ertapenem， imipenem or meropenem. According to the modified carbapenem inactivation test of CLSI M100S-27th file， it was confirmed that they were enzyme-producing strains. Five carbapenemase genes （bla KPC、bla IMP、bla OXA-48、bla VIM、bla NDM）were amplified by PCR. PCR amplified products were sequenced and genotypes were identified. The homology of Klebsiella pneumoniae was analyzed by pulsed field gel electrophoresis. Results：Thirty-four strains of Klebsiella pneumoniae were confirmed to be enzyme-producing strains by modified carbapenem inactivation test， and all of them were KPC-2 enzyme by PCR detection. PFGE results showed that 34 strains could be divided into 5 groups， of which 1， 2 and 4 were the main epidemic strains. Conclusion：CR-KP clones with KPC-2 as the main drug resistance gene were prevalent in the hospital， and drug-resistant strains were prevalent in the department of critical medicine. There were 34 strains with different degrees of genetic relationship.