重组酿酒酵母表达白斑综合征病毒VP28的保护性初探

重组酿酒酵母表达白斑综合征病毒VP28的保护性初探

张炜,岑黔鸿,高彤,雷涵

西南交通大学医学院,西南交通大学医学院,西南交通大学医学院,西南交通大学医学院

收稿日期:2019-04-09 修回日期:2019-04-09 出版日期:2019-04-25
基金资助:
中央高校基本科研业务费专项资金资助（项目编号：2682018CX73；项目名称：基于酿酒酵母表面展示系统构建寨卡口服疫苗的免疫活性研究）；四川省科技计划项目(项目编号：2019YFN0134；项目名称：酿酒酵母表面展示技术应用于高致病性禽流感口服疫苗的研究)

Preliminary Study on Protective Efficacy of Recombinant Saccharomyces cerevisiae Expressing VP28 of White Spot Syndrome Virus

Zhang Wei,Cen QianHong,Gao Tong and Lei Han

College of Medicine, Southwest Jiaotong University,College of Medicine, Southwest Jiaotong University,College of Medicine, Southwest Jiaotong University,College of Medicine, Southwest Jiaotong University

Received:2019-04-09 Revised:2019-04-09 Online:2019-04-25

摘要/Abstract

摘要： 目的：构建重组酿酒酵母表达白斑综合征病毒VP28蛋白，并对其口服给药后的保护效率进行分析。方法：以白斑综合征病毒的vp28基因（基因库编号： FJ756456.1）作为研究对象，构建重组酿酒酵母S. cerevisiae EBY100/pYD1-VP28，通过SDS-PAGE、Western blot以及免疫荧光分析对重组酿酒酵母S. cerevisiae EBY100/pYD1-VP28的表达进行检测。以白肢虾（Penaeus vannamei）作为动物模型，通过白斑综合征病毒攻击实验，检测重组酿酒酵母S. cerevisiae EBY100/pYD1-VP28的保护效率。结果：口服重组酿酒酵母EBY100/pYD1-VP28后的对虾存活率为60%。结论：重组酿酒酵母S. cerevisiae EBY100/pYD1-VP28可以应用于对虾养殖业预防白斑综合征病毒的感染，潜藏着巨大的市场应用前景。

Abstract: Objective：To construct recombinant Saccharomyces cerevisiae（S. cerevisiae） expressing VP28 antigen protein of white spot syndrome virus(WSSV) and investigate its protective efficacy after oral administration. Methods：Recombinant S. cerevisiae EBY100/pYD1-VP28 was constructed based on vp28 gene of WSSV （gene accession No.FJ756456.1）as research subject and analyzed by SDS-PAGE， Western blot and immunofluorescence assay.Further， Penaeus vannamei was used as an animal model， protective efficacy of recombinant S. cerevisiae EBY100/pYD1-VP28 was determined by WSSV challenge. Results：Oral administration of recombinant S. cerevisiae EBY100/pYD1-VP28 could provide 60% of survival rate in Penaeus vannamei. Conclusion：Recombinant S. cerevisiae EBY100/pYD1-VP28 can prevent WSSV infection in shrimp culture industry and has a great potential for market.

张炜,岑黔鸿,高彤,雷涵. 重组酿酒酵母表达白斑综合征病毒VP28的保护性初探[J]. .

Zhang Wei,Cen QianHong,Gao Tong and Lei Han. Preliminary Study on Protective Efficacy of Recombinant Saccharomyces cerevisiae Expressing VP28 of White Spot Syndrome Virus[J]. .

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