Abstract: Objective：To establish a method for the determination of chlorogenic acid content in jinyin qingwen mixture. Methods： Water decoction and distillation were used to extract the effective components of the medicinal materials. HPLC method was used to determine the content of chlorogenic acid， Use Diamonsil G8 ODS column (4.6 nm×200 mm， 5 μm)， taking 50% methanol-0.4% phosphoric acid water as mobile phase，the flow rate was 1.0 mL?min-1， column temperature was 30 ℃， detection wave length was 327 nm， sample quantity was 10 μL. Results： Chlorogenic acid retention time was 18.126 min， linear range was 0.991-9.93(r=0.999 9)， average recovery was 99.16%， detection limit was 0.1 μg?mL-1. Conclusion： The preparation process of jinyin qingwen mixture is stable.HPLC method for determination of chlorogenic acid content is efficient， accurate and stable， and the quality standard is feasible.