关键词: 壮腰健身丸, 超高效液相色谱, 多波长切换法, 多指标含量测定

Abstract:

Objective： To establish an ultra-high performance liquid chromatography（UPLC） method for the determination of multiple index components such as salidroside， 2，3，5，4'-tetrahydroxystilbene-2-O-β-D-glucoside， triligustrine and emodin in Zhuangyao Jianshen Pills.Methods： Waters Acquity UPLC^TM Tss T3 column （1.8 μm，100 × 2.1 mm） was used. Methanol（A）-0.1% formic acid solution（B） was used as mobile phase，gradient elution. The flow rate was 0.2 mL·min^-1. The detection wavelength was 224 nm， 254 nm， 288 nm and 320 nm.Results： There were good linearity in the ranges of 0.762 0～30.48 μg·mL^-1（r=0.999 9）for salidroside， 0.611 0～24.46 μg·mL^-1（r=1.000 0）for 2，3，5，4'-tetrahydroxystilbene-2-O-β-D-glucoside， 3.001～120.0 μg·mL^-1（r=0.999 9）for specnuezhenide and 0.338 0～13.50 μg·mL^-1（r=0.999 9） for emodin .The average recoveries were 96.6%， 96.6%， 96.2%， 100.4%， and the RSDs were all less than 2.0% （n=6）.Conclusion： The established method realizes the simultaneou determination of multiple-index components in Zhuangyao Jianshen Pills. The method has strong specificity， good repeatability and high accuracy， and which provides reliable technical support for ensuring drug quality and improving medication safety.

Key words: Zhuangyao Jianshen pills； , Ultra performance liquid chromatography（UPLC）； , Multi-wavelength switching method； , Multi-index content determination