Abstract: Objective：To establish a method on the content determination of water-soluble polysaccharide in fructus ligustri lucidi. Methods: Both anthranone-sulfuric acid colorimetry and UV visible spectrophotometry were used, which the blank solution of the sample was used as reference and glucose solution as the control sample. Results: Under the wavelength of 570 nm, when glucose reference substance was 0-0.1 mg·mL-1, the linear relationship between the absorbance and the concentration was good. When glucose (0.1 mg·mL-1) as the reference substance, the linear regression line equation was A=0.992 3C+0.004 2(r=0.999 2, n=6). The content of water-soluble polysaccharide was 8.03 mg·g-1, the average recovery was 100.54%, RSD was 2.80%(n=6), and the precision was good. Using anthranone-sulfuric acid colorimetry to determine the content of water-soluble polysaccharide in fructus ligustri lucidi, fructus ligustri lucidi water-soluble polysaccharide and glucose reference substance determined by ultraviolet wavelength scanning all have maximum absorbance at 570 nm, and the methodological indicators such as linearity, stability, precision and recovery were performing well. Conclusion: The method is simple, feasible, and suitable for determining the content of water-soluble polysaccharide in fructus ligustri lucidi.