Abstract: Objective: To investigate the ICU mainly infected with resistant strains of pathogenic and non-ICU analysis,provide a scientific basis for the prevention of ICU infections.Methods:ICU patients and non-ICU patients admitted to our hospital in January 2008～January 2012 a total of 598 cases including 362 cases of male patients,236 cases of female patients,age range 21～67 years old,average age of 38.4 years.218 cases of all patients,surgical patients,medical patients(n= 156),224 cases of obstetrics and gynecology patients.ICU patients 161 cases,437 cases of non-ICU patients.Random hospital in ICU and ward patients and hospitalized patients in the general ward strain collection,microbiology routine bacterial culture,isolation and identification in the month of January 2008 2012 1 period.The collected samples of bacteria in ICU patients and non-ICU patients with urine,blood,sputum,wound and patients with various discharge,ICU in addition to air,ground,pipelines,water,apparatus,equipment,etc.sample collection.The above three types of bacterial samples were named as the ICU patient group,the ICU ward group,non-ICU group.The above three groups of samples for analysis of drug resistance.Results:The pathogens are mainly distributed in the patient’s sputum,wound around the ICU instrument equipment surfaces.Pathogen resistance in ICU group was significantly higher than the other two groups,drug-resistant pathogens,followed by group of ICU patients,non-ICU group of pathogens resistant to the weakest.Conclusion:ICU pathogen resistance is too high during treatment need to select the lower drug resistance to anti-infective,and strengthen the management of the hospital in ICU to prevent the abuse of antibiotics.