Abstract: Objective：To evaluate the clinical effect of ultrasound-guided cervical lymph nodes biopsy on diagnosis.Methods：Randomly selected 75 cases from April 2015 to April 2016 in our hospital for ultrasound-guided biopsy of the cervical lymph nodes， and clinical data were retrospectively analyzed.Results：A total of 240 strip of tissue were taken from 75 patients， of which 222 strips fulfilled pathological requirements， and the success rate of puncture biopsy was 92.5%.All patients had no obvious adverse reactions，and there was nearly no bleeding after compression.Pathology showed 74 cases were diagnosed correctly，1 case was not determined， until histological examination after operation confirmed it schwannoma. Among those diagnosed patients，70.7% were lymph node malignant disease，of which 10 were lymphoma and 43 were metastatic carcinoma； 22 cases were benign lesions，accounting for 29.3%， mainly for lymphoid tuberculosis and lymphadenitis. The accuracy of ultrasonography and ultrasound-guided biopsy in diagnosis of benign lesions subjected to pathological reports was 77.3% and 97.7%，and accuracy of malignant lesions was 93.4% and 100% respectively.The overall diagnostic accuracy was 88.7% and 99.3%， and the accuracy of ultrasound-guided biopsy was significantly higher than that of ultrasonography (P<0.05). Conclusion： The clinical effect of ultrasound-guided cervical lymph node biopsy in diagnosis is satisfactory，which is suitable for a wide range of clinical application.