基于网络药理学和分子对接技术的祛瘀生新方治疗脑梗死作用机制研究
收稿日期: 2024-09-29
修回日期: 2025-06-14
录用日期: 2025-08-02
网络出版日期: 2025-12-17
基金资助
江苏省自然科学基金青年项目(BK20251142);江苏省中医院新技术新项目(2023DNP09);江苏省中医院院级课题(Y19044,Y20025,y2023cx06);江苏省中医药管理局课题(MS2022016);江苏省卫生健康委课题(M2024078)
Research on the Mechanism of Action of Quyu Shengxin Formula in Treating Cerebral Infarction Based on Network Pharmacology and Molecular Docking
Received date: 2024-09-29
Revised date: 2025-06-14
Accepted date: 2025-08-02
Online published: 2025-12-17
Supported by
基于周细胞PDGFRβ信号研究通脑饮“祛瘀生新”促进缺血性脑卒中后血管新生的作用机制研究(MS2022016)
李昀泽
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基于网络药理学和分子对接技术的祛瘀生新方治疗脑梗死作用机制研究
[1] 贾建平,陈生弟,崔丽英.神经病学[M].北京:人民卫生出版社,2018:194-195.
[2] 《中国脑卒中防治报告2021》编写组.《中国脑卒中防治报告2021》概要[J].中国脑血管病杂志,2023,20(11):783-793.
[3] Zhao Y, Hua X, Ren X, et al. Increasing burden of stroke in China: a systematic review and meta-analysis of prevalence, incidence, mortality, and case fatality[J].Int J Sroke, 2023,18(3):259-267.
[4] Ma Q, Li R, Wang L, et al. Temporal trend and attributable risk factors of stroke burden in China, 1990-2019: an analysis for the global burden of disease study 2019[J].Lancet Public Health, 2021,6(12):e897-e906.
[5] 郝雅文.急性脑梗死的治疗进展[J].天津药学,2021,33(4):66-69.
[6] 李昀泽,姚阳婧,冯清华,等.首都国医名师田从豁教授针药结合六经分治中枢神经系统感染临床经验探析[J].四川中医,2024,42(9):1-7.
[7] 李昀泽,姚阳婧,陈安琪,等.中医心理沙盘可应用在抗击新型冠状病毒感染肺炎医务人员心理问题的治疗[J].四川中医,2020,38(3):9-12.
[8] 沈璐莹,徐红,李宏辉,等.协定方通脑饮治疗痰瘀阻络型急性脑梗死疗效观察[J].北京中医药,2021,40(10):1058-1060.
[9] 陈兆耀,夏鑫,姚蓓蓓,等.急性脑梗死病人阿替普酶溶栓后24 h内使用通脑饮治疗的安全性和有效性分析[J].中西医结合心脑血管病杂志,2020,18(13):2147-2149.
[10] 朱元,常诚,王欣彤.通脑饮治疗急性脑梗死的临床疗效及对患者血液流变学影响[J].河北医学,2018,24(10):1639-1643.
[11] 张珂.颈动脉支架置入术联合通脑饮治疗痰瘀阻络型认知功能障碍的临床疗效评价[D].南京:南京中医药大学,2022.
[12] 蒋怡雯.基于外周血NLR及PLR评价通脑饮治疗急性缺血性卒中的临床疗效及预后[D].南京:南京中医药大学,2022.
[13] 王雨轩.通脑饮治疗缺血性脑卒中的疗效观察及其干预斑马鱼血管损伤模型的转录组学研究[D].南京:南京中医药大学,2021.
[14] 徐红.痰瘀阻络型急性脑梗死中认知障碍的评估与协定方通脑饮的干预研究[D].南京:南京中医药大学,2021.
[15] Ru J, Li P, Wang J, et al. TCMSP: a database of systems pharmacology for drug discovery from herbal medicines[J].J Cheminform, 2014,6:13.
[16] 柏林宇,曾惠娜,巫秀美,等.康复新液促进创伤修复的网络药理学研究[J].广东化工,2022,49(8):55-58.
[17] Safran M, Dalah I, Alexander J,et al. GeneCards version 3: the human gene integrator[J].Database (Oxford), 2010:baq020.
[18] Amberger JS, Bocchini CA, Schiettecatte F, et al.OMIM.org: online mendelian inheritance in man(OMIM), an online catalog of human genes and genetic disorders[J].Nucleic Acids Res, 2015, 43(Database issue):789-798.
[19] Szklarczyk D, Gable AL, Lyon D, et al. STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets[J].Nucleic Acids Res, 2019,47(D1):D607-D613.
[20] Huang DW, Sherman BT, Lempicki RA. Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists[J].Nucleic Acids Res, 2009,37(1):1-13.
[21] Ghosh P, Beas AO, Bornheimer SJ, et al. A Gαi-GIV molecular complex binds epidermal growth factor receptor and determines whether cells migrate or proliferate[J].Mol Biol Cell, 2010,21(13):2338-2354.
[22] Meier TI,Cook JA,Thomas JE, et al. Cloning, expression, purification, and characterization of the human Class Ia phosphoinositide 3-kinase isoforms[J].Protein Expr Purif, 2004,35:218-224.
[23] Maheshwari S, Miller MS, O'Meally R, et al. Kinetic and structural analyses reveal residues in phosphoinositide 3-kinase α that are critical for catalysis and substrate recognition[J].J Biol Chem, 2017,292(33):13541-13550.
[24] Feigin VL, Krishnamurthi RV, Parmar P, et al. Update on the global burden of ischemic and hemorrhagic stroke in 1990-2013: the GBD 2013 study[J].Neurpepidemiology, 2015,45(3):161-176.
[25] 陈孝男,杨爱琳,赵亚楠,等.缺血性脑中风的发病机制及其常用治疗中药研究进展[J].中国中药杂志,2019,44(3):422-432.
[26] 张艾嘉,王爽,王萍,等.缺血性脑卒中的病理机制研究进展及中医药防治[J].中国实验方剂学杂志,2020,26(5):227-240.
[27] Natalie A, Hermanns IM, Andreas S,et al. Renin, aldosterone, the aldosterone-to-renin ratio, and incident hypertension among normotensive subjects from the general population[J].Cardiovasc Res, 2022,119(1):294-301.
[28] Isordia-Salas I, Santiago-Germán D, Cerda-Mancillas MC, et al. Gene polymorphisms of angiotensin-converting enzyme and angiotensinogen and risk of idiopathic ischemic stroke[J].Gene,2019,688:163-170.
[29] Werden E, Khlif MS, Bird LJ, et al. APOE ɛ4 carriers show delayed recovery of verbal memory and smaller entorhinal volume in the first year after ischemic stroke[J].J Alzheimers Dis, 2019,71(1):245-259.
[30] Yue YH, Bai X, Zhang H, et al. Gene polymorphisms affect the effectiveness of atorvastatin in treating ischemic stroke patients[J].Cell Physiol Biochem, 2016,39(2):630-638.
[31] Jayaraj RL, Azimullah S, Beiram R, et al. Neuroinflammation: friend and foe for ischemic stroke[J].J Neuroinflammation, 2019,16(1):142.
[32] Kim JT, Lee JS, Kim H,et al. Comparative effectiveness of rosuvastatin versus atorvastatin in acute ischemic stroke treatment[J].J Am Heart Assoc, 2025,14(3):e038080.
[33] Takahashi G, Watanabe T, Satoh T .Infective endocarditis caused by streptococcus sanguinis resulting in stroke, ruptured infected pseudoaneurysm of superior mesenteric artery, and rapidly progressive glomerulonephritis[J].Internal Med, 2024,63(3):413-147.
[34] Mu J, Zou X, Bao X, et al.bFGF-Chitosan "brain glue" promotes functional recovery after cortical ischemic stroke[J].Bioact Mater, 2025,46:386-405.
[35] 中国中西医结合学会神经科专业委员会.中国脑梗死中西医结合诊治指南(2017)[J].中国中西医结合杂志,2018,38(2):136-144.
[36] Kotb HG, Ibrahim AH, Mohamed EF, et al. The expression of microRNA 146a in patients with ischemic stroke: an observational study[J].Int J Gen Med,2019,12:273-278.
[37] Gu J, Gui S, Hu L, et al. Downregulated miRNA-324-5p aggravates neuronal injury induced by oxygen-glucose deprivation via modulating RAN[J].Exp Ther Med, 2020,19(1):658-664.
[38] Wang J, Wu M. The up-regulation of miR-21 by gastrodin to promote the angiogenesis ability of human umbilical vein endothelial cells by activating the signaling pathway of PI3K/Akt[J].Bioengineered, 2021,12(1):5402-5410.
[39] 李昀泽,姚阳婧,陈安琪,等.中医心理沙盘治疗新型冠状病毒感染肺炎合并焦虑抑郁患者374例临床疗效观察[J].医学食疗与健康,2020,18(24):208-209.
[40] 李昀泽,陈伟伟,李建军,等.协定方通脑饮治疗急性缺血性卒中临床疗效及安全性评价[J].辽宁中医药大学学报,2025,27(9):143-148.
[41] Jiang H, Wang J, Sheng L, et al. Tongnao decoction (TND) alleviated atherosclerosis by playing lowering lipid, anti-inflammatory, and antioxidant roles[J].Oxid Med Cell Longev, 2022,2022:6061197.
[42] Liu B, Neufeld AH. Activation of epidermal growth factor receptors in astrocytes: from development to neural injury[J].J Neurosci Res, 2007,85(16):3523-3529.
[43] Ciccolini F, Mandl C, Hölzl-Wenig G, et al. Prospective isolation of late development multipotent precursors whose migration is promoted by EGFR[J].Dev Biol, 2005,284(1):112-125.
[44] Thorpe LM, Yuzugullu H, Zhao JJ. PI3K in cancer: divergent roles of isoforms, modes of activation and therapeutic targeting[J].Nat Rev Cancer, 2015,15(1):7-24.
[45] Wee P, Wang Z. Epidermal growth factor receptor cell proliferation signaling pathways[J].Cancers, 2017,9(5):52.
[46] Barteczek P, Li L, Ernst AS, et al. Neuronal HIF-1α and HIF-2α deficiency improves neuronal survival and sensorimotor function in the early acute phase after ischemic stroke[J].J Cereb Blood Flow Metab, 2017,37(1):291-306.
[47] 蒋瑞冲,王琦伟,徐敉,等.急性缺血性脑卒中发病早期血清脑红蛋白和 HIF-1α水平变化及意义[J].医学研究杂志,2018,47(4):125-129.
[48] 姚辉,舒涛,徐义春,等.姜黄素通过激活cAMP-PKA-CREB信号通路促进诱导多功能干细胞神经分化[J].中国中西医结合杂志,2021,41(5):602-607.
[49] 王建丽.通脑饮激活PI3K/Akt信号通路对缺血性脑卒中后血管生成的机制研究及临床意义[D].南京:南京中医药大学,2022.
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