CHINESE JOURNAL OF MEDICINAL GUIDE ›› 2025, Vol. 27 ›› Issue (12): 1321-1321-1326.doi: magtech.2024.12.05-00003

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Effects of Qiqi Yishen Capsules on Podocyte Pyroptosis in Diabetes Nephropathy Rats Based on the NLRP3/GSDMD Pathway

LIU Xinghua, WANG Shirong, ZHANG Jinzhen, GUO Erhua, ZHAO Yang   

  1. Shanxi Provincial Hospital of Traditional Chinese Medicine Shanxi Taiyuan 030012, China
  • Received:2024-12-05 Revised:2025-10-28 Accepted:2025-12-24 Online:2025-12-28 Published:2026-01-26

Abstract:

Objective: To investigate the effect of Qiqi Yishen capsules on inhibiting podocyte pyroptosis through NLRP3/ GSDMD pathway and delaying the progression of diabetic nephropathy.Methods: By establishing diabetes nephropathy DN rat model 48 SD rats were randomly divided into five groups with 12 rats in each group which were the blank group the model group benazepril positive control group Qiqi Yishen capsule treatment group. Compare and analyze the levels of 24-hour urinary protein blood urine nitrogen BUN), blood creatinine SCr), triglyceride TG) ,total cholesterol TC of the rats and renal pathological morphology was observed. IL-1β and IL-18 were detected by ELISA.The expression of GSDMD protein in rat kidney was detected by immunohistochemistry. The expressions of NLRP3 Caspase-1and Nephrin in renal tissues were detected by Western blot.Results: Compared with the blank group the levels of urinary protein blood lipid TG TC)、 BUN SCr increased in the model group. Meanwhile there was capillary loop hypertrophy of glomerular tissue in renal tissue. Mesangial matrix increased significantly and basement membrane thickened. The levels of IL-1β and IL-18 and the expressions of GSDMD NLRP3 and Caspase-1 increased and the expression of podocyte marker Nephrin decreasesd P<0.01. Compared with the model group urine protein blood lipid BUN SCr decreased in the treatment group. At the same time mesangial matrix decreased. The levels of IL-1β and IL-18 decreased. The expressions of GSDMD NLRP3 and Caspase-1 decreased and the level of Nephrin protein recovered in the treatment groups.Conclusion: Qiqi yishen capsules may inhibit podocyte pyroptosis reduce proteinuria and delay the progression of kidney disease by regulating NLRP3/GSDMD pathway.

  

Key words: Diabetes nephropathy , Pyroptosis , NLRP3 , Qiqi yishen capsules , Podocyte

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