Abstract: Objective:To study the effect of using computer virtual reality simulation for percutaneous renal puncture training.Methods:According to the clinical experience,trainees were divided into 3 groups.89 urologists without experience of percutaneous renal puncture,27 surgical residents and masters,38 clinical students accepted the training with virtual reality simulation.Results:In 3 groups,there were significant differences in success rate of percutaneous renal puncture with virtual reality simulation after training(P<0.05).We compared 3 groups’ scores.Before training,the scores of urologists were significant differences better than other 2 groups.After training,the scores of urologists and surgical residents were no significant differences(P>0.05),these 2 groups’ scores were significant differences better than clinical students(P<0.05).Conclusion:The training of percutaneous renal puncture with virtual reality simulation was beneficial to all the trainees.In this study,we found the better training effect was gained on trainees of clinical experience.