Abstract: 【ABSTRACT】Objective: To explore the mammography guided wire localization operation biopsy technique in non palpable breast lesions (NPBL) and the clinical value of the application of skills. Methods: 64 cases of molybdenum target X-ray on NPBL patients, using two-dimensional positioning device in X-ray guided percutaneous indwelling wire hook, surgical cutting biopsy in wire guide. With rapid histopathologic specimens were performed intraoperative radiographic examination, according to the pathological results of surgery or surgery decided to end. Results: 64 cases of patients with accurate positioning, complete resection of lesions. The pathological results of 50 cases of benign lesions, 14 cases of breast cancer, including 7 cases of ductal carcinoma in situ, underwent breast conserving surgery for breast in 3 cases, total resection and sentinel lymph node biopsy in 4 cases, 6 cases of infiltrating ductal carcinoma, breast conserving surgery in 2 cases, 4 cases of modified radical mastectomy, 1 cases of medullary carcinoma modified radical operation. Conclusion: Reasonable positioning and cutting wire inspection techniques can improve the accuracy of resection of the lesion, found the breast X-ray BI-RADS class 4 and 5 NPBL steel wire positioning operation biopsy can identify the nature of the lesion, improve the detection rate of early breast cancer.