黄芩汤颗粒剂中黄芩苷、甘草酸及芍药苷的含量测定研究

黄芩汤颗粒剂中黄芩苷、甘草酸及芍药苷的含量测定研究

刘亚萍,陈佩东,曹雨诞

江苏省中医院,南京中医药大学药学院,南京中医药大学药学院

收稿日期:2017-04-20 修回日期:2017-08-13 出版日期:2017-08-25
基金资助:
南京中医药大学风险基金(No.CX201202)；江苏高校优势学科建设项目（PAPD， No. ysxk-2014)

Content Determination of Baicalin，Glycyrrhizic Acid and Paeoniflorin in Huangqin Decoction Granule

刘亚萍,chen peidong and cao yudan

Jiangsu province hospital of traditional Chinese medicine,College of Nanjing university of Chinese medicine,College of Nanjing university of Chinese medicine

Received:2017-04-20 Revised:2017-08-13 Online:2017-08-25

摘要/Abstract

摘要： 目的：测定黄芩汤颗粒剂主要成分的含量。方法：采用高效液相色谱法测定处方黄芩中的黄芩苷，甘草中的甘草酸和白芍中的芍药苷含量，以Kromasil C18（4.6 mm×250 mm，5 μm）色谱柱，应用 0.05%磷酸和乙腈梯度洗脱并进行方法学考察。结果：高效液相色谱法测出在黄芩苷3.6~57.60 μg·mL-1，r=0.9996；甘草酸在4.16~52 μg·mL-1，r=0.9999；芍药苷在2.85~22.8 μg·mL-1，r=0.9999 范围内呈良好的线性关系，黄芩苷、甘草酸及芍药苷的平均回收率分别为 96.31%，97.69%和97.27%；RSD分别为1.29%，1.03% 和1.29%。结论：建立的含量测定方法简便、重现性好，可用于黄芩汤颗粒剂的质量控制。

Abstract: Objective： To establish a method of content determination on baicalin， glycyrrhizic and paeoniflorin of huangqin decoction granule.Methods： The content of baicalin，glycyrrhizin and paeoniflorin was measured by HPLC， the column was Kromasil C18 (4.6 mm×250 mm， 5 μm)， the mobile phase was selected 0.05% phosphoric acid and acetonitrile with a gradient elution. Results：The linear ranges of baicalin，glycyrrhizin and paeoniflorin were within 3.6~57.60 μg·mL-1， r=0.9996； 4.16~52 μg·mL-1， r=0.9999； 2.85~22.8 μg·mL-1， r=0.9999，and the average recoveries were 96.31% (RSD of 1.29 %)， 97.69% (RSD of 1.03 %) and 97.27% (RSD of 1.29 %). Conclusion： The method is simple and reproducible， and can be used for the quality control of Huangqin decoction granule.

刘亚萍,陈佩东,曹雨诞. 黄芩汤颗粒剂中黄芩苷、甘草酸及芍药苷的含量测定研究[J]. .

刘亚萍,chen peidong and cao yudan. Content Determination of Baicalin，Glycyrrhizic Acid and Paeoniflorin in Huangqin Decoction Granule[J]. .

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