HPLC法测定地锦草中游离和总没食子酸含量

HPLC法测定地锦草中游离和总没食子酸含量

邬秋萍,万林春

江西省药品检验检测研究院,江西省药品检验检测研究院

收稿日期:2017-08-02 修回日期:2017-08-02 出版日期:2017-09-25
基金资助:
江西省科技计划项目(项目编号：20151BBG70040；项目名称：肠炎宁片中地锦草炮制工艺及其质量标准研究)

Quantitative Determination of Free Gallic Acid and Total Gallic Acid in Euphorbiae Humifusae Herba by HPLC

WU Qiu-ing and WAN Lin-chun

Jiangxi Provicial Research Institute for Drug Control,Jiangxi Provicial Engineering Research Center for Drug and Medical Device Quality,Jiangxi Provicial Research Institute for Drug Control,Jiangxi Provicial Engineering Research Center for Drug and Medical Device Quality

Received:2017-08-02 Revised:2017-08-02 Online:2017-09-25

摘要/Abstract

摘要： 目的：建立HPLC法测定地锦草中游离没食子酸和总没食子酸的含量。方法：采用Agela Odyssil C18（4.6 mm×250 mm，5 μm）色谱柱；流动相为甲醇-0.5%磷酸溶液（8∶92）；检测波长270 nm；流速1.0 mL·min-1；柱温30 ℃。结果：没食子酸在0.034 0~1.700 2 μg范围内呈良好线性关系（r=1.000 0），游离没食子酸和总没食子酸平均回收率分别为99.63%和99.34%。结论：该方法操作简便准确，灵敏度高，重复性好，可用于地锦草中没食子酸的质量控制。

Abstract: Objective：To establish a method for quantitative determination of free gallic acid and total gallic acid in Euphorbiae Humifusae Herba by HPLC.Methods：The HPLC procedure was performed on the chromatographic column of Agela Odyssil C18（4.6 mm×250 mm， 5μm）at 30℃， the mobile phase was methanol-0.5% phosphoric acid solution（8∶92）with the flow velocity of 1.0 mL·min-1， and the detection wavelength was 270 nm.Results： Gallic acid showed a good linear relationship with peak area at a range from 0.0340 μg to 1.7002 μg(r= 1.0000)， the free gallic acid recovery was 99.63% and the total gallic acid recovery was 99.34%.Conclusion：The method is simple， accurate with high sensitivity and good repeatability， and can be used for the quality control of gallic acid in Euphorbiae Humifusae Herba.

邬秋萍,万林春. HPLC法测定地锦草中游离和总没食子酸含量[J]. .

WU Qiu-ing and WAN Lin-chun. Quantitative Determination of Free Gallic Acid and Total Gallic Acid in Euphorbiae Humifusae Herba by HPLC[J]. .

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