南药益智仁对糖尿病肾病氧化应激iNOS及PARLmRNA影响的实验研究

南药益智仁对糖尿病肾病氧化应激iNOS及PARLmRNA影响的实验研究

韦袆,谢毅强,罗嘉莉,李泗平,李姗柳,梁薇

海南医学院,海南医学院,海南医学院,海南医学院,海南医学院,广西中医药大学

收稿日期:2018-12-20 修回日期:2018-12-20 出版日期:2018-12-25
基金资助:
国家自然科学基金项目(项目编号：81360586；项目名称：益智仁-乌药调控线粒体氧化应激治疗肾阳虚型糖尿病肾病的机制研究)；国家自然科学基金项目（项目编号：81473618；项目名称：益智仁乌药调控线粒体氧化应激治疗肾阳虚型糖尿病肾病的机制研究)；海南省自然科学基金项目(项目编号：812186；项目名称：南药益智仁对糖尿病肾病AQP2及P27kipl的影响 )；海南省中药现代化专项(项目编号：ZY201332；项目名称：南药益智仁调控糖尿病肾病TLR信号途径基因甲基化的研究)；海口市重点科技项目(项

Effect of Bitter Cardamon on the Expression of iNOS and PARLmRNA in Oxidative Stress of Diabetic Nephropathy

WEI Yi,XIE Yiqiang,LUO Jiali,LI Siping,LI Shanliu and LIANG Wei

Hainan Medical College,Hainan Medical College,海南医学院,海南医学院,海南医学院,广西中医药大学

Received:2018-12-20 Revised:2018-12-20 Online:2018-12-25

摘要/Abstract

摘要： 目的：探讨益智仁对STZ造模的糖尿病肾病（diabetic nephropathy，DN）大鼠治疗后的血清诱生型一氧化氮合酶(iNOS)及早老素相关菱形样蛋白(PARL)mRNA表达的影响，以期确定益智仁的抗氧化应激作用。方法：大鼠高糖高脂饮食联合链脲佐菌素（STZ）腹腔注射造模4周。大鼠随机分为6组，每组10 只，分别为空白组，益智仁低、中、高剂量组（低、中、高剂量分别为100、300、900 mg·kg-1），缬沙坦组（缬沙坦胶囊25 mg·kg-1·d-1），模型组。给药4周后用酶联免疫吸附实验(ELISA)法测血清iNOS，荧光定量PCR法测PARLmRNA。结果：益智仁不同浓度药物均能下调血清iNOS及增加PARLmRNA的表达（P<0.05）。结论：益智仁能通过干预iNOS及PARLmRNA的表达治疗氧化应激引起的DN。

Abstract: Objective：To investigate the changes of serum inducible nitric oxide synthase(iNOS) and presenilin-associated rhomboid-like protein(PARL) mRNA after the treatment of STZ-induced rat models of diabetic nephropathy（DN） with bitter cardamon and to determine the antioxidative stress effect of bitter cardamon Methods： Rats were treated with high glucose and high-fat diet combined with intraperitoneal injection of STZ for 4 weeks. Rats were randomly divided into 6 groups，10 in each group，which were the blank group， the low， medium and high dose group of bitter cardamon(low，medium and high dose were 100， 300， 900 mg·kg-1 respectively）， valsartan group (valsartan capsules， 25 mg·kg-1·d-1)， the model group respectively. After 4 weeks， serum iNOS was measured by ELISA method， and PARLmRNA was detected by fluorescence quantitative PCR method. Results： The different concentrations of the drug could down-regulate serum iNOS and increase the expression of PARLmRNA（P<0.05）. Conclusion： The extract of bitter cardamon can treat oxidative stress induced DN by the intervention of expression of iNOS and PARLmRNA.

韦袆,谢毅强,罗嘉莉,李泗平,李姗柳,梁薇. 南药益智仁对糖尿病肾病氧化应激iNOS及PARLmRNA影响的实验研究[J]. .

WEI Yi,XIE Yiqiang,LUO Jiali,LI Siping,LI Shanliu and LIANG Wei. Effect of Bitter Cardamon on the Expression of iNOS and PARLmRNA in Oxidative Stress of Diabetic Nephropathy[J]. .

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