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紫花地丁与混淆品分类鉴别的探索性研究

陈叶青,金佩芬,俞秀,金小燕,李建国,张纯,宋喆旎,屠斌华,沈春燕   

  1. 嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院
  • 收稿日期:2018-07-06 修回日期:2018-07-26 出版日期:2018-07-25
  • 基金资助:
    嘉兴市科技局项目(项目编号:SQ2017000720;项目名称:紫花地丁及混伪品鉴定方法和安全性指标研究)

Exploratory Research on the Identification of Herba Violae and its Adulterants

CHEN Yeqing,JIN Peifen,YU Xiu,JIN Xiaoyan,LI Jianguo,ZHANG Chun,SONG Zheni,TU Binhua and SHEN Chunyan   

  1. jiaxing food and drug testing institute,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院,嘉兴市食品药品检验检测院
  • Received:2018-07-06 Revised:2018-07-26 Online:2018-07-25

摘要: 目的:考察市售紫花地丁质量,寻求鉴别紫花地丁的专属性方法。方法:采用性状观察、薄层色谱法、高效液相色谱方法进行比较鉴别。结果:紫花地丁与同科同属植物在性状、薄层上较难区分,通过液相方法,其色谱图有明显差异,可以作为新的鉴别方法将紫花地丁与其他混淆品区分。结论:本实验提供的鉴别方法,能将紫花地丁与其他5个易混品种加以区别。

Abstract: Objective:To investigate the quality of the commercially available herba violae and to seek the method of identifying the specificity of herba violae.Methods: The characters observation, thin layer chromatography(TLC) and high-performace liquid chromatography(HPLC) were used for comparative identification. Results:It was difficult to distinguish herba violae and plants of the same genus of the same family by characters and TLC.Through HPLC, there were obvious differences in the chromatogram, and it could be used as a new identification method to distinguish herba violae from other adulterants.Conclusion: The method of identification provided in this experiment can distinguish herba violae from five other easily confnsed varieties.