关键词: font-size:medium, ">UPLC-MS/MS；参芪健胃颗粒；芍药苷；橙皮苷；甘草酸铵

Abstract: Objective： To establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for determination of paeoniflorin， hesperdidin， ammonium glycyrrhizinate in shenqi jianwei granules. Methods：The determination was performed on Agilent Eclipse Plus C18 column (2.1 mm×50 mm，1.8 μm)， with the mobile phase consisted of acetonile-0.1% formic acid (gradient elution) at the fluid speed of 0.3 mL·min-1. The column temperature was set at 35 ℃. Multiple reaction monitoring (MRM) in negative mode was used for the quantification of paeoniflorin， hesperidin， ammonium glycyrrhizinate， using chloramphenicol as an internal standard (IS). Results：The liner range of paeoniflorin， hesperidin， ammonium glycyrrhizinate were 0.044-11.24， 0.021-10.924， 0.016-5.275 μg·mL-1， respectively (r>0.999 0). The limits of quantitation were 11.0， 7.0， 8.0 ng·mL-1. The limits of detection were 4.4， 3.5， 4.0 ng·mL-1 respectively. The results demonstrated satisfying precision， repeatability， and stability. Average recoveries were 95%～105% (RSD<6.5%， n=6). Conclusion：The established UPLC-MS/MS method is accurate， simple， sensitive and specific， which can be used for determination of paeoniflorin， hesperidin， ammonium glycyrrhizinate in shenqi jianwei granules.
  

Key words: font-size:medium, ">UPLC-MS/MS； Shenqi jianwei granules；Paeoniflorin； Hesperidin； Ammonium glycyrrhizinate