关键词: font-size:medium, ">焦白术；白术内酯Ⅰ、Ⅱ、Ⅲ；质量标准；HPLC；TLC

Abstract: Objective： To establish quality standards of deep-fried Atractylodes macrocephala. Methods： The characters and microscopic characteristics of the decoction pieces of deep-fried Atractylodes macrocephala were described by means of morphological and microscopic identification. The contents of water、total ash and extractum were analysed according to the methods of Chinese Pharmacopoeia(2015). Qualitative identification was by thin layer chromatography (TLC). The content of atractylenolideⅠ、atractylenolide Ⅱ and atractylenolide Ⅲ were analysed by HPLC method.The determination was performed on a C18 column(4.6 mm×250 mm，5 μm) with acetonitrile-water as the mobile phase. The flow rate was 1.0 mL·min-1； column temperature was 30 ℃. The detection wavelength was 220 nm and 276 nm. Results： The microscopic features are obvious and the specificity was good. TLC method has clear spots and good separation degree .The average contents of water was 3.2%， total ash was 4.3%， extractum was 71.8%； The content of atractylenolideⅠ、atractylenolide Ⅱ and atractylenolide Ⅲ were 120.1， 136.8， 162.5 μg·g-1 respectively.Adjacent peaks were completely separated and has a good linear relationship， the sample recovery （n=6）were 99.9%，93.6%， 91.2% respectively，RSD was 1.3%，2.1%，2.6% respectively. Conclusion：This established method is simple， feasibleand specific，and can be effectively suitable for the quality deep- fried Atractylodes macrocephala.，and improve the quality standards.

Key words: font-size:medium, ">Deep-fried Atractylodes macrocephala； AtractylenolideⅠ、 Atractylenolide Ⅱ and Atractylenolide Ⅲ； Quality standard； HPLC； TLC