关键词: font-size:medium, ">miRNA；lncRNA；NK细胞；树突状细胞；胃癌细胞

Abstract: Objective： To study the molecular mechanism of miRNA-lncRNA interaction affecting the immune function of human NK cells and dendritic cells（DC） and the invasiveness of gastric cancer （GC）cells. Methods： The experiment was divided into 4 groups： GC tissue， normal tissue， GC cell line， normal cell line. lncRNA (MEG3) was detected by qRT-PCR in 4 experimental groups. NK cell and DC cell viability were detected by CCK-8. Migration and invasion of GC cells were measured by Transwell assay. Relationship between miRNA (miR-21) and MEG3 were detected by luciferase activity. After miR-21 mimic transfection or MEG3 transfection， the expression levels of NK cell immune function marker protein (CD56)， DC cell immunological function marker protein (CD80) and cell invasion marker proteins (MMP-2 and MMP-9) were detected by protein immunoassay. Results： We found that MEG3 is down-regulated expressed in GC tissues and cells(P<0.05). Overexpression of MEG3 significantly inhibited NK cell and DC cell viability and the migration and invasion of GC cells (P<0.05). Overexpression of miR-21 resulted in MEG3-WT luciferase activity significantly decreased， but had no significant effect on the luciferase activity of the MEG3-mut reporter gene. Overexpression of MEG3 attenuated the expression of CD56， CD80， MMP-2 and MMP-9 proteins by miR-21 mimics (P<0.05) . Conclusion： Study reveals that miRNA-lncRNA interactions regulate the immune function of human NK cells and DC cells and the progression GC cell invasion. It induces the overexpression of MEG3 and inhibites NK cell and DC cell viability.

Key words: font-size:medium, ">miRNA； lncRNA；Natural killer cells； Dendritic cells； Gastric cancer cells