miR-184对胃癌细胞增殖和迁徙的作用研究

摘要/Abstract

摘要： 目的：探究miR-184对胃癌细胞增殖和迁徙的作用及其机制。方法：利用real-time PCR技术检测人胃癌组织及癌旁组织中miR-184的含量，继而检测正常胃黏膜细胞及多种胃癌细胞系中miR-184的含量；利用miR-184抑制剂（miR-184抑制剂组）和miR-184阴性对照序列（对照组）分别转染胃癌细胞系MNK-45后，利用CCK-8法检测该细胞系增殖能力变化；利用划痕实验和Transwell实验检测细胞的迁徙能力；利用Western Blot技术检测细胞Ki67、上皮性钙黏附蛋白（E-cadherin）、神经性钙黏附蛋白（N-cadherin）表达水平。结果：与癌旁组织相比，胃癌组织中miR-184高表达（P<0.05）；与正常胃黏膜细胞相比，多种胃癌细胞系中miR-184高表达（P<0.05）；与对照组相比，miR-184抑制剂能够显著下调胃癌细胞系MNK-45内miR-184水平（P<0.05），并抑制MNK-45细胞增殖和迁徙（P<0.05）；与对照组相比，miR-184抑制剂降低了Ki67和N-cadherin的表达（P<0.05），提高了E-cadherin的表达（P<0.05）。结论：胃癌组织及胃癌细胞系内高表达miR-184，下调miR-184可抑制胃癌细胞的增殖和迁徙。

关键词: font-size:medium, ">胃癌；增殖；迁徙；miRNA ,

Abstract: Objective： To explore the effect of miR-184 on the proliferation and migration of gastric cancer cells. Methods： Real-time PCR was used to detect the miR-184 level in human gastric cancer tissues and adjacent tissues， as well as in normal gastric mucosa cells and various gastric cancer cell lines. Gastric cancer cell line MNK-45 were transfected with miR-184 inhibitor (inhibitor group) and miR-184 negative control sequence (control group). CCK-8 assay was used to detect the cell proliferation. Scratch test and Transwell test were used to detect cell migration. The protein levels of Ki67， E-cadherin and N-cadherin were detected by Western Blot. Results： The level of miR-184 in gastric cancer tissues was higher than that in adjacent tissues (P<0.05). Compared with normal gastric mucosa cells， miR-184 was highly expressed in many gastric cancer cell lines (P<0.05). Compared with the control group， miR-184 inhibitor significantly decreased the level of miR-184 in gastric cancer cell line MNK-45 (P<0.05). Compared with the control group， miR-184 inhibitor suppressed the proliferation and migration of MNK-45 (P<0.05). Moreover， miR-184 inhibitor decreased the expression of Ki67 and N-cadherin（P<0.05）， but increased the expression of E-cadherin（P<0.05）. Conclusion： miR-184 is highly expressed in gastric cancer tissues and gastric cancer cell lines. Down regulation of miR-184 may inhibit the proliferation and migration of gastric cancer cells.

Key words: font-size:medium, ">Gastric cancer； Proliferation； Migration； miRNA

中图分类号:

侯花屏, 李庆. miR-184对胃癌细胞增殖和迁徙的作用研究[J]. 中国医药导刊, 2021, 23(5): 371-375.

HOU Huaping, LI Qing. The Effect of miR-184 on the Proliferation and Migration of Gastric Cancer Cells[J]. CHINESE JOURNAL OF MEDICINAL GUIDE, 2021, 23(5): 371-375.

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