关键词: font-size:medium, ">高效液相色谱-荧光检测法；六神曲（炒）；黄曲霉毒素；免疫亲和柱；光化学衍生

Abstract: Objective：To establish a high performance liquid chromatography-fluorescence detection (HPLC-FLD)method for simultaneous determination of aflatoxin B1，B2，G1 and G2 in Massa Medicata Fermentata (Fried).Methods： After extraction with 70% methanol，purification by immunoaffinity column，high performance liquid chromatography and post-column photochemical derivatization，the content of aflatoxin B1，B2，G1 and G2 in the samples were determined by fluorescence detector. Results：The linearity range of aflatoxin B1 was 0.0104-0.0520 ng（r=0.9998）.The linearity range of aflatoxin B2 was 0.0038-0.0190 ng（r=0.9991）.The linearity range of aflatoxin G1 was 0.0108-0.0540 ng（r=0.9991）.The linearity range ofaflatoxin G2 was 0.0038-0.0190 ng（r=0.9993）.The linear relationship was good.The recoveries were within 80.54%-91.37％， with RSD≤3.5%. Conclusion：The method established has the advantages of convenient operation， high sensitivity， good repeatability and accurate results.Aflatoxin G2 was detected in 10 batches of Massa Medicata Fermentata (Fried).This method is suitable for the determination of aflatoxin in Massa Medicata Fermentata (Fried).

Key words: font-size:medium, ">HPLC-FLD；Massa Medicata Fermentata (Fried)；Aflatoxins；Immunoaffinity column；Photochemical derivatization