关键词: font-size:medium, ">侧柏叶配方颗粒；高效液相色谱法；指纹图谱；质量研究

Abstract: Objective：To establish an HPLC fingerprint spectrum of formula granule of Cacumen Platycladi， to provide experimental evidence for its quality research evaluation. Methods：Take 20% methanol extract of Cacumen Platycladi formula granules as sample by ultrasonic processing. HPLC analysis was carried out on Agilent ZORBAX SB-C18 (4.6 mm×250 mm，5 μm) chromatographic column with mobile phase of acetonitrile-1% glacial acetic acid， gradient elution with a flow at 1.0 mL·min-1， an ultraviolet detection wavelength at 254 nm，the column temperature at 30 ℃. The injection volume of reference substance was 10 μL， the injection volume of test substance was 20 μL， and the recording time was 50 min. Results：The RSD of the precision， stability and repeatability test of Cacumen Platycladi formula granules were 0.03%， 0.06%， 0.21%， all of which were not more than 2.0%（n=6）. The chromatograms obtained by gradient elution have good separation results and meet the requirements of fingerprints spectrums. A systematic comparison with the fingerprints spectrums of Cacumen Platycladi crude drug， decoction pieces and decoction showed that the similarity of each batch of samples was high， and the uniformity was good. All showed 13 common peaks， and the relative retention time of each common peak was stable. Conclusion： The method is accurate，credible with good repeatability， which it can provide reference evidence for quality control of Cacumen Platycladi formula granules.

Key words: font-size:medium, ">Cacumen Platycladi formula granule； HPLC； Fingerprint spectrum； Quality research