circDLGAP4及miR-34c在帕金森病患者血清中的表达及临床意义

摘要/Abstract

摘要： 目的：探讨环状RNA-DLGAP4 （circular RNA-DLGAP4，circDLGAP4）及微小RNA-34c （microRNA-34c，miR-34c）在帕金森病（Parkinson′s disease，PD）患者血清中的表达及其对疾病的诊断价值。方法：选取2019年1月至2021年1月我院收治的72例PD患者作为PD组，并根据Hoehn-Yahr分级对PD患者进行分组。另选取同期在我院体检的健康志愿者75例作为对照组。通过实时荧光定量PCR检测两组受试者血清circDLGAP4及miR-34c的相对表达量，并通过统一帕金森病评定量表（UPDRS）统计PD患者UPDRS Ⅰ、UPDRS Ⅱ及UPDRS Ⅲ评分。通过Pearson相关性、受试者工作特征（ROC）曲线分析circDLGAP4及miR-34c在PD中的诊断价值。结果：PD组患者血清circDLGAP4和miR-34c表达水平低于对照组（P＜0.05）。中度组PD患者血清circDLGAP4和miR-34c表达水平较轻度组更低（P＜0.05）；重度组PD患者血清circDLGAP4和miR-34c表达水平较中度组更低（P＜0.05）。PD患者血清circDLGAP4和miR-34c表达水平与UPDRS Ⅱ评分、UPDRS Ⅲ评分、Hoehn-Yahr分级呈负相关（P＜0.05）。血清circDLGAP4、miR-34c联合检测对PD的诊断效能更高。结论：circDLGAP4及miR-34c可作为诊断PD的分子标志物。

关键词: font-size:medium, ">帕金森病；circDLGAP4；miR-34c；血清；诊断价值；生物标志物

Abstract: Objective：To investigate the expression and diagnostic value of circDLGAP4 and miR-34c in the serum of Parkinson′s disease (PD) patients. Methods：72 PD patients admitted to our hospital from January 2019 to January 2021 were selected as the PD group， and were grouped according to Hoehn-Yahr grade. In addition， 75 healthy volunteers who underwent physical examination in our hospital during the same period were selected as the control group. The relative expression levels of circDLGAP4 and miR-34c in the serum of the two groups of subjects were detected by real-time quantitative PCR， and the UPDRS Ⅰ， UPDRS Ⅱ and UPDRS Ⅲ scores of PD patients were calculated by the Unified Parkinson′s Disease Rating Scale (UPDRS). The diagnostic value of circDLGAP4 and miR-34c in PD was analyzed by the method of Person correlation and receiver operating characteristic (ROC) curve. Results：The serum circDLGAP4 and miR-34c expression levels in the PD group were lower than those in the control group (P<0.05). The serum circDLGAP4 and miR-34c expression levels in moderate group Parkinson′s disease patients were lower than those in the mild group (P<0.05). The serum circDLGAP4 and miR-34c expression levels in severe group Parkinson′s disease patients were lower than those in the moderate group (P<0.05). The serum circDLGAP4 and miR-34c expression levels in PD patients were negatively correlated with UPDRS Ⅱ score， UPDRS Ⅲ score and Hoehn-Yahr grade (P<0.05). The combined detection of serum circDLGAP4 and miR-34c is more valuable in the diagnosis of PD. Conclusion：circDLGAP4 and miR-34c can be used as the molecular markers to diagnose PD.

Key words: font-size:medium, ">Parkinson′s disease； circDLGAP4； miR-34c； Serum； Diagnostic value； Biomarkers

中图分类号:

翟海燕, 杨杰. circDLGAP4及miR-34c在帕金森病患者血清中的表达及临床意义[J]. 中国医药导刊, 2023, 25(3): 281-285.

ZHAI Haiyan, YANG Jie. Expression and Clinical Significance of circDLGAP4 and miR-34c in the Serum of Patients with Parkinson′s Disease[J]. CHINESE JOURNAL OF MEDICINAL GUIDE, 2023, 25(3): 281-285.

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