关键词: font-size:medium, ">黄曲霉毒素； 炒僵蚕； 免疫亲和柱-高效液相色谱法

Abstract: Objective： Immunoaffinity chromatography-high performance liquid chromatography (IAC-HPLC) was used to detect the aflatoxin B1， B2， G1 and G2 in fired Bombyx batryticatus， so as to provide reference for the improvement of the quality standard of fired Bombyx batryticatus and its market supervision. Methods： The analysis of 7 batches of fried Bombyx batryticatus was performed on a YMC-C18 (250 mm×4.6 mm， 5 μm) column with methanol-acetonitrile-water (32∶18∶50) as the mobile phase at a flow rate of 1.0 mL·min-1. The column temperature was 30 ℃. The excitation and emission wavelengths of the fluorescence detector were 360 nm and 450 nm， respectively. Results： The detection methods of aflatoxin B1， B2， G1 and G2 adopted in this study showed good linearity， precision and recovery . Aflatoxin G2 was detected in multiple batches of samples， with a detection rate of 86 %. The content of aflatoxin G1 in one batch of samples was higher. According to the limit standard of aflatoxin in Chinese Pharmacopoeia 2020 edition， the total amount of aflatoxin B2， G1 and G2 exceeded the limit. Conclusion： This study is the first time to detect aflatoxin in fried Bombyx Batrvticatus bimmunoaffinity column-high performance liquid chromatography, and to clarify the safety risks otaflatoxin in fried Bombyx Batryticatus, so as to provide reference for the quality control of aflatoxirin fried Bombyx Batryticatus.

Key words: font-size:medium, ">Aflatoxin； Fried Bombyx batryticatus； IAC-HPLC