关键词: font-size:medium, ">复方硫酸软骨素片；硫酸软骨素；芍药苷；甘草酸；梯度洗脱

Abstract: Objective： To establish an HPLC gradient elution method for simultaneous determination of chondroitin sulfate， paeoniflorin and glycyrrhizic acid in compound chondroitin sulfate tablets. Methods： The chromatographic separation was performed on InertSustain C18 column（4.6 mm×250 mm，5 μm），and gradient elution was conducted with 5 mmol·L-1 sodium heptanesulfonate solution as mobile phase A and acetonitrile as mobile phase B. The column temperature was 30℃， and the detection wavelength was 230nm. Results： The linear ranges of chondroitin sulfate， paeoniflorin and glycyrrhizic acid were 0.292 3-7.307 6， 0.002 6-0.132 0 and 0.006 1-0.301 8 mg·mL-1， respectively. The average recoveries were 98.5%-99.7%， and RSD was 1.0%-1.2%. The content ranges of chondroitin sulfate， paeoniflorin and glycyrrhizic acid in 4 batches of samples were 72.954 7-74.103 6， 0.661 3-0.689 8 and 1.502 3-1.520 9 mg per tablet， respectively. Conclusion： The HPLC gradient elution method has been established for simultaneous determination of the contents of three active components in compound chondroitin sulfate tablets. The method is simple， rapid and accurate， and suitable to be used for the quality control of compound chondroitin sulfate tablets.

Key words: font-size:medium, ">Compound chondroitin sulfate tablets； Chondroitin sulfate； Paeoniflorin； Glycyrrhizic acid； Gradient elution