湿热体质人群肠道菌群分布特征分析

中国医药导刊 ›› 2023, Vol. 25 ›› Issue (7): 733-738.

湿热体质人群肠道菌群分布特征分析

李剑莹1，莫秀云1，韦芳宁2*

1.广州中医药大学第二附属医院，广东广州 510120；
2.广东省中医院治未病中心，广东广州 510120

收稿日期:2023-06-15 修回日期:2023-06-30 出版日期:2023-07-28 发布日期:2023-07-28
基金资助:
广东省中医院名中医药专家学术经验传承工作室建设项目（项目编号：E43713；项目名称：王琦学术经验传承工作室）

Analysis of the Distribution Characteristics of Intestinal Flora in the Population with Damp-heat Constitution

1.The Second Affiliated Hospital of Guangzhou University of Chinese Medicine， Guangdong Guangzhou 510120， China；
2.Preventive Treatment Center， Guangdong Provincial Hospital of Chinese Medicine， Guangdong Guangzhou 510120， China

Received:2023-06-15 Revised:2023-06-30 Online:2023-07-28 Published:2023-07-28

摘要/Abstract

摘要： 目的：探讨湿热体质人群肠道菌群分布特征。方法：选取2021年１月至2022年12月于广东省中医院二沙岛医院体检科体检的湿热体质人群30例纳入湿热质组；另选取同期在医院体检的平和体质人群30例纳入平和质组。收集两组人群粪便，通过16S rDNA高通量测序，并采用生物信息学方法对测序结果进行处理与分析，比较湿热质与平和质两种人群肠道菌群结构的不同之处。结果：湿热质组及平和质组的肠道菌群存在明显差异，湿热质组的Shannon指数低于平和质组（P<0.05）。主坐标分析 (principal co-ordinates analysis，PCoA)显示，湿热质组和平和质组的肠道菌群结构分布差异较大（P<0.05）。门分类水平上，湿热质组拟杆菌门/厚壁菌门相对丰度比值呈下降趋势，疣微菌门的相对丰度低于平和质组（P<0.01）。属分类水平上，湿热质组中布劳特氏菌属、迟缓埃格特菌的相对丰度均高于平和质组(P<0.05)，双歧杆菌属、Alistipes、Odoribacter、瘤胃球菌属、颤螺菌属、无色杆菌属、Anaerostipes的相对丰度均低于平和质组(P<0.05)。结论：湿热体质人群存在肠道菌群紊乱。

关键词: font-size:medium, ">湿热质；平和质；肠道菌群；分布特征；生物信息学

Abstract: Objective：To explore the distribution characteristics of intestinal flora in people with damp-heat constitution. Methods：30 people with damp-heat constitution who had physical examination in Ersha Island Hospital of Guangdong Provincial Hospital of Chinese Medicine from January 2021 to December 2022 were selected as the damp-heat constitution group， and 30 people with normal constitution were selected as the normal constitution group. The stool samples were taken from the two groups of people. 16S rDNA high-throughput sequencing was performed， and the sequencing results were processed and analyzed by bioinformatics method to find the differences in intestinal microflora structure between the two groups of people. Results：There were obvious differences in intestinal flora between the damp-heat constitution group and the normal constitution group. The Shannon index of the damp-heat constitution group was lower than that of the normal constitution group (P<0.05). Principal Co-ordinates Analysis showed that the intestinal flora structure distribution of the damp-heat constitution group and the normal constitution group was different(P<0.05). At the phylum classification level，the relative abundance ratio of Bacteroidetes and Firmicutes in the damp-heat constitution group showed a decreased trend， and the relative abundance of Verrucomicrobia in the damp-heat constitution group was lower than that of the normal constitution group(P<0.01). At the genus level，the relative abundances of Blautia，Eggerthella of the damp-heat constitution group were higher than those of the normal constitution group (P<0.05)，and the relative abundance of Bifidobacterium， Alistipes， Odoribacter， Ruminococcus， Oscillospira， Achromobacter， Anaerostipes of the damp-heat constitution group were lower than those of the normal constitution group (P<0.05).Conclusion：Gut microbiota imbalance exist in people with damp-heat constitution.

Key words: font-size:medium, ">Damp-heat constitution；Normal constitution；Intestinal flora；Distribution characteristics；Bioinformatics

中图分类号:

李剑莹, 莫秀云, 韦芳宁. 湿热体质人群肠道菌群分布特征分析[J]. 中国医药导刊, 2023, 25(7): 733-738.

LI Jianying, MO Xiuyun, WEI Fangning. Analysis of the Distribution Characteristics of Intestinal Flora in the Population with Damp-heat Constitution[J]. CHINESE JOURNAL OF MEDICINAL GUIDE, 2023, 25(7): 733-738.

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