关键词: font-size:medium, ">艾纳香；生态型；樟脑；L-龙脑；β-石竹烯

Abstract: Objective： To establish a GC method for content determination of bornanone， L-borneol and β-caryophyllene in Blumea balsamifera (B. balsamifera)， and to determine the contents of the above three components in 4 ecotypes of B. balsamifera at five life stage which was planted in Guizhou Wangmo county. Methods： The chromatographic separation was performed on HP-5 capillary column（30 m×0.32 mm， 0.25 μm）， temperature programmed， and the inlet temperature was set at 200 ℃. The temperature of the detector was controlled at 280 ℃ and the injection volume was 1 μL with split injection. Results and Conclusion： The established method is sample， accurate and repeatable， and can be used for content determination of bornanone， L-borneol and β-caryophyllene in B. balsamifera. The contents of three component were varied in different ecotypes at various life stages. Contents of L-borneol “in horse” ear type and large-leaf type were higher than other two ecotypes. Contents of bornanone was lower in lobular type， while higher “in horse” ear type. Contents of β-caryophyllene was higher in wild type and lobular type than in other two ecotypes. According to the quality requirements of Pharmacopoeia of the People′s Republic of China， it is suggested that when use B. balsamifera as the raw material to extract sage parder， it is preferable to cultivate large-leaf type， and it should be collected for extraction in late November.

Key words: font-size:medium, ">Blumea balsamifera； Ecotypes； Bornanone； L-borneol； β-caryophyllene