miR-1301通过靶向p53促进胃癌细胞的增殖

中国医药导刊 ›› 2021, Vol. 23 ›› Issue (12): 926-932.

miR-1301通过靶向p53促进胃癌细胞的增殖

贺栋伟1，郭明飞2*，石菲3

1.榆林市第一医院普外二科，陕西榆林 718000； 2.榆林市第四（星元）医院普外一科，陕西榆林 719000； 3.空军军医大学航空航天医学院，陕西西安 710032

收稿日期:2021-08-24 修回日期:2021-09-23 出版日期:2021-12-28 发布日期:2021-12-28
基金资助:
国家自然科学基金项目(项目编号：31971171；项目名称：模拟失重下BMECs外泌体源性lncRNA ODSM介导成骨细胞cyclin B1泛素化降解的机制研究)

miR-1301 Promotes the Proliferation of Gastric Cancer Cells by Targeting p53

1.Department of General Surgery, the First Hospital of Yulin, Shaanxi Yulin 718000, China; 2.Department of General Surgery, the Fourth Hospital of Yulin/Xingyuan Hospital of Yulin, Shaanxi Yulin 719000, China; 3.School of Aerospace Medicine, Air Force Medical University, Shaanxi Xi′an 710032, China

Received:2021-08-24 Revised:2021-09-23 Online:2021-12-28 Published:2021-12-28

摘要/Abstract

摘要： 目的:探究miR-1301对胃癌细胞增殖的作用及其机制。方法：利用real-time PCR技术检测人胃癌组织及癌旁组织中miR-1301的含量，继而检测正常胃黏膜细胞及多种胃癌细胞系中miR-1301的含量；利用miR-1301的抑制剂（抑制剂组）和miR-1301的阴性对照序列（对照组）分别转染胃癌细胞系MNK-45后，利用EdU染色法和CCK-8法检测该细胞系增殖能力变化；利用Western Blot技术检测细胞磷脂酰肌醇3激酶/蛋白质丝氨酸苏氨酸激酶（phosphatidylinositol-3-kinases/protein-serine-threonine kinase, PI3K/Akt）蛋白表达水平。利用荧光素酶报告基因技术检测miR-1301的作用靶点。结果：与癌旁组织相比，胃癌组织中miR-1301高表达（P<0.05）；与正常胃黏膜细胞相比，多种胃癌细胞系中miR-1301高表达（P<0.05）；与对照组相比，miR-1301抑制剂能够下调胃癌细胞系MNK-45内miR-1301水平（P<0.05），并抑制MNK-45细胞增殖（P<0.05）；与对照组相比，miR-1301抑制剂能够抑制MNK-45细胞PI3K/Akt信号通路的活化（P<0.05）。与对照组相比，miR-1301能够抑制抑癌基因p53 3′UTR质粒的荧光素酶活性（P<0.05）。结论：胃癌组织及胃癌细胞系内高表达miR-1301，miR-1301可通过靶向p53促进胃癌细胞的增殖。

关键词: font-size:medium, ">胃癌；增殖；miRNA；PI3K/Akt信号通路；p53

Abstract: Objective:To explore the effect and mechanism of miR-1301 on the proliferation of gastric cancer cells. Methods: Real time PCR was used to detect the miR-1301 level in human gastric cancer tissues and adjacent tissues, as well as in normal gastric mucosa cells and various gastric cancer cell lines. Gastric cancer cell line MNK-45 were transfected with miR-1301 inhibitor(the inhibitor group) and miR-1301 negative control sequence(the control group) respectively.EdU staining and CCK-8 assay were used to detect the cell proliferation.The protein expression levels of PI3K/Akt(phosphatidylinositol-3-kinases/protein-serine-threonine kinase) were detected by Western Blot. The target of miR-1301 was detected by luciferase reporter genetechnology. Results: The level of miR-1301 in gastric cancer tissues was higher than that in adjacent tissues(P<0.05).Compared with normal gastric mucosa cells, miR-1301 was highly expressed in many gastric cancer cell lines(P<0.05).Compared with the control group, miR-1301 inhibitor significantly decreased the level of miR-1301 in gastric cancer cell line MNK-45(P<0.05). Compared with the control group, miR-1301 inhibitor suppressed the proliferation of MNK-45 and PI3K/Akt signaling pathway activation in MNK-45(P<0.05). miR-1301 mimics inhibited luciferase activity of the 3′UTR plasmid of p53 compared with the control group(P<0.05). Conclusion: MiR-1301 is highly expressed in gastric cancer tissues and gastric cancer cell lines. miR-1301 may promote the proliferation of gastric cancer cells by targeting p53.

Key words: Gastric cancer, Proliferation, miRNA, PI3K/Akt signaling pathway;p53

中图分类号:

贺栋伟, 郭明飞, 石菲. miR-1301通过靶向p53促进胃癌细胞的增殖[J]. 中国医药导刊, 2021, 23(12): 926-932.

HE Dongwei, GUO Mingfei, SHI Fei. miR-1301 Promotes the Proliferation of Gastric Cancer Cells by Targeting p53[J]. CHINESE JOURNAL OF MEDICINAL GUIDE, 2021, 23(12): 926-932.

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