关键词: font-size:medium, ">瓜蒌皮；瓜蒌籽；UPLC-Triple TOF-MS/MS；差异成分分析

Abstract: Objective：To analyze the chemical constituents of pericarpium trichosanthis and semen trichosanthis.Methods:Ultra-performance liquid chromatography-Triple TOF-MS/MS(UPLC-Triple TOF-MS/MS) was used to determine the chemical constituents of pericarpium trichosanthis and semen trichosanthis from the same source. The characteristic peaks were extracted by peak matching， peak alignment and noise filtering through two-stage tandem mass spectrometry analysis. Principal component analysis(PCA) and OPLS-DA analysis were used for data processing. According to the accurate mass charge ratio of the first order mass spectrometry and the fragment information of the second order mass spectrometry， the compositions were identified by software database search and related literature.Results:The chemical constituents of fructus pericarpium trichosanthis and semen trichosanthis were significantly different from those of pericarpium trichosanthis and semen trichosanthis from the same source were effectively distinguished. Twenty-six different chemical components were screened and identified， 20 of which were common and showed different variation patterns. Conclusion:From the perspective of chemical composition difference of pericarpium trichosanthis and semen trichosanthis， it can provide basis for revealing the material basis of drug property and efficacy difference of pericarpium trichosanthis and semen trichosanthis， and also provide scientific basis for the development and utilization of pericarpium trichosanthis and semen trichosanthis.

Key words: Pericarpium trichosanthis;Semen trichosanthis;UPLC-triple TOF-MS/MS, Composition difference analysis