关键词: 舒肝和胃丸, α-香附酮, 香附烯酮, HPLC-UV法, 含量测定

Abstract:

Objective： To establish a content determination method of high performance liquid chromatography （HPLC-UV） for α-cyperone and cyperenone in shugan hewei pills， and to provide a basis for quality control of the content of vinegar-processed Cyperi Rhizoma in this preparation.Methods： The content of α-cyperone and cyperenone in shugan hewei pills were determined simultaneously. The analysis was performed on a Agilent ZORBAX SB-C₁₈ chromatography column （4.6 mm × 250 mm， 5 μm），with mobile phase consisting of methanol and water （65∶35） at the flow rate of 1.0 mL·min^-1. The detection wavelength was 254 nm. The column temperature was 30 ℃， and the injection volume was 5 μL.Results： In the chromatogram of shugan hewei pills obtained by this method， α-cyperone and cyperenone have good separation effects from adjacent chromatographic peaks， meeting the requirements for content determination.The linear ranges of indicator components α-cyperone and cyperenone were 0.080 36 - 0.803 60 µg and 0.211 2 - 2.112 0 μg， respectively. The linear relationship within the range is good， and the correlation coefficients were all 1.000. The average spiked recovery rates were 102.0% （RSD： 1.6%， n=6） and 102.4% （RSD： 2.0%， n=6）， respectively. The stability （24 h）， repeatability and precision were compliance with requirements.Conclusion： The established method to determin the content of α-cyperone and cyperenone by HPLC-UV is simple， fast and accurate， and can provide a basis for controlling the content of vinegar-processed Cyperi Rhizoma in shugan hewei pills.

Key words:  , Shugan hewei pills； , α-cyperone； , Cyperenone； , HPLC-UV method； , Content determination