关键词: 原子荧光光谱法, 活血止痛胶囊, 砷, 铅, 汞

Abstract:

Objective： To establish an atomic fluorescence spectrometry method for determining the content of arsenic， lead and mercury in Huoxue Zhitong capsules.Methods： The samples were digested by microwave digestion method. The content of arsenic， lead and mercury in 6 batches of Huoxue Zhitong capsules were determined by hydride generation - atomic fluorescence spectrometry.Results： Arsenic， lead and mercury showed good linear relationships within the ranges of 0-20， 0-10 and 0-2 μg·L^-1 respectively， and the correlation coefficients （r） were all ≥ 0.999 8. The limit of detection （LOD） for arsenic， lead， and mercury were 0.056 9 μg·L^-1， 0.047 6 μg·L^-1 and 0.005 2 μg·L^-1 respectively. The spike recoveries were all between 82.0% and 108.9%. The RSDs of the determination precision of arsenic， lead and mercury were 1.6%， 1.4% and 2.0% respectively. The RSDs of the stability within 8 hours were 3.9%， 3.7% and 4.9% respectively. The RSDs of repeatability were 2.5%， 1.6% and 3.7% respectively.Conclusion： Atomic fluorescence spectrometry can accurately determine the contents of arsenic， lead and mercury in Huoxue Zhitong capsules. This method is simple， stable and reliable， and has good methodological validation results. It can be used as a new method for the determination of heavy metals in Huoxue Zhitong capsules， and also provides a new idea for the limit control of arsenic， lead and mercury elements in other Chinese patent medicines.

Key words:  , Atomic fluorescence spectrometry； Huoxue Zhitong capsules； Arsenic； Lead； Mercury