Abstract: [Abstract] Objective: To study the analysis of different periods of different types of clinical specimens of microbiological test results positive rate distribution. Methods: To review 2000 cases of test specimens of our hospital in January 2012 January ~2013 and March 2013 ~2014 year in March period, and the standard of microbiological examination for clinical analysis, the positive rate of different periods and different types clinical samples for microbiological examination results. Results: the January 2012 ~2013 year in January the respiratory tract specimens and other non respiratory specimens for microbiological testing positive rate low, the higher the positive rate of blood culture samples, March 2013 ~2014 year in March the respiratory tract and other non respiratory tract microbial test positive rate is higher, the positive rate of blood culture were lower, between the two groups compared to P<0.05,.2012 was statistically significant in January ~2013 year in January the specimen test results the positive rate of ~2014 in March and March 2013 The results of stool samples testing positive rate comparison, P>0.05, no statistical significance. Conclusion: different periods of different types of clinical specimens of microbiological test results positive rate differences, more to be based on different clinical samples for microbiological examination positive rate, to find reasons, provide the scientific basis for disease diagnosis and treatment.