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中国医药导刊 ›› 2022, Vol. 24 ›› Issue (3): 219-221.

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雷公藤基因组及其活性成分生物合成途径解析

高伟1,2   

  1. 1.首都医科大学附属北京世纪坛医院,北京 100038;  2.首都医科大学中医药学院,北京 100069
  • 收稿日期:2022-04-25 出版日期:2022-03-26 发布日期:2022-03-28

Analysis of the Tripterygium wilfordii Genome and the Biosynthetic Pathway of its Active Natural Products

  1. 1.Beijing Shijitan Hospital, Capital Medical University, Beijing 100038,China; 2.School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • Received:2022-04-25 Online:2022-03-26 Published:2022-03-28

摘要: 近年来,中药雷公藤因富含多种活性成分而受到广泛关注,其二萜类成分雷公藤甲素因其显著的抗癌活性更是成为研究新热点。在黄璐琦院士的指导下,本课题组致力于雷公藤的基因组学研究,并结合代谢组、转录组数据,克隆鉴定雷公藤甲素生物合成途径上的关键酶基因,解析其关键生源途径,并利用合成生物学技术在酵母中重构相关中间体的代谢途径,实现雷公藤甲素生物合成途径关键中间体的异源合成。该研究成果对于发掘和保护我国中药基因资源及其活性成分的合成生物学生产具有重大科学意义和应用价值,并荣获“2020年度中医药十大学术进展”。

关键词: font-size:medium, ">雷公藤;基因组;雷公藤甲素;生物合成途径;异源生产

Abstract: In recent years, the Chinese medicine Tripterygium wilfordii has received widespread attention due to its richness in various active ingredients. One of its diterpenoids components, triptolide, has become a hot research object due to its remarkable anticancer activity. Under the guidance of Academician Huang Luqi, our research group worked on the genomic study of Tripterygium wilfordii, combining metabolomic and transcriptomic data to clone and identify key enzyme genes and resolve key biosynthetic pathway of triptolide. We further used synthetic biology techniques to reconstruct the metabolic pathway of related intermediates in yeast to achieve the heterologous bioproduction of key intermediates in triptolide biosynthesis. The research results have great scientific significance and application value for the discovery and conservation of genetic resources of Chinese medicine and synthetic biology production of its active natural products, and were awarded the Top 10 Academic Advances in Chinese Medicine in 2020.

Key words: font-size:medium, ">Tripterygium wilfordii;Genome;Triptolide; Biosynthetic pathway; Heterologous bioproduction

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