关键词: 2, 3, 5, 4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷；HPLC；制何首乌；不确定度

Abstract: Objective： To evaluate the uncertainty of 2， 3， 5， 4′-tetrahydroxystilbene-2-O-β-D-glucos ide in processed polygonum multiflorum by HPLC， in order to provide reference for comprehensive evaluation of the accuracy of analytical methods. Methods： By establishing a mathematical model for the determination of 2， 3， 5， 4′-tetrahydroxystilbene-2-O-β-D-glucos ide.The factors of influencing the uncertainty was analyzed and evaluated for each factor.The Agilent ZORBAX SB-C18（4.6 mm×250 mm，5 μm）was used.The mobile phase consisted of acetonitrile and purified water was 25∶75 and at a flow rate of 1.0 mL·min-1. The injection volume of was 10 μL. The column temperature was set at 30 ℃ and detection wavelength was 320 nm. Results： The RSD of the repeatability test of 2， 3， 5， 4′-tetrahydroxystilbene-2-O-β-D-glucos ide was 0.76% （n=6）， the average recovery was 99.2%（RSD=0.84％， n=9）. With confidence probability （P）95%， the expanded uncertainty of 2， 3， 5， 4′-tetrahydroxystilbene-2-O-β-D-glucos ide in processed polygonum multiflorum was 0.02%. Conclusion： The uncertainty analysis method is applicable to evaluation of uncertainty in measurement of 2， 3， 5， 4′-tetrahydroxystilbene-2-O-β-D-glucos ide in processed polygonum multiflorum.
  

Key words: 2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucos ide； HPLC； Processed polygonum multiflorum； Uncertainly